This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2jmh
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | Blo t 5 is the major allergen from Blomia tropicalis mites and shows | + | Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases. |
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
| - | Roles of | + | Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18184590 18184590] |
[[Category: Blomia tropicalis]] | [[Category: Blomia tropicalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 17: | Line 17: | ||
[[Category: Huang, T.]] | [[Category: Huang, T.]] | ||
[[Category: Kuo, I.]] | [[Category: Kuo, I.]] | ||
| - | [[Category: Naik, M | + | [[Category: Naik, M T.]] |
[[Category: allergen]] | [[Category: allergen]] | ||
[[Category: blo t 5]] | [[Category: blo t 5]] | ||
| Line 24: | Line 24: | ||
[[Category: group 5]] | [[Category: group 5]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:04:03 2008'' |
Revision as of 16:04, 21 February 2008
|
NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis
Overview
Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases.
About this Structure
2JMH is a Single protein structure of sequence from Blomia tropicalis. Full crystallographic information is available from OCA.
Reference
Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:18184590
Page seeded by OCA on Thu Feb 21 18:04:03 2008
Categories: Blomia tropicalis | Single protein | Chang, C. | Chua, K. | Huang, T. | Kuo, I. | Naik, M T. | Allergen | Blo t 5 | Dust mites | Group 5
