2jnc
From Proteopedia
(New page: 200px<br /><applet load="2jnc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jnc" /> '''Refined 3D NMR structure of ECD1 of mCRF-R2b...) |
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| - | [[Image:2jnc.jpg|left|200px]]<br /><applet load="2jnc" size=" | + | [[Image:2jnc.jpg|left|200px]]<br /><applet load="2jnc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2jnc" /> | caption="2jnc" /> | ||
'''Refined 3D NMR structure of ECD1 of mCRF-R2beta at pH 5'''<br /> | '''Refined 3D NMR structure of ECD1 of mCRF-R2beta at pH 5'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The corticotropin releasing factor (CRF) family of ligands and their | + | The corticotropin releasing factor (CRF) family of ligands and their receptors coordinate endocrine, behavioral, autonomic, and metabolic responses to stress and play additional roles within the cardiovascular, gastrointestinal, and other systems. The actions of CRF and the related urocortins are mediated by activation of two receptors, CRF-R1 and CRF-R2, belonging to the B1 family of G protein-coupled receptors. The short-consensus-repeat fold (SCR) within the first extracellular domain (ECD1) of the CRF receptor(s) comprises the major ligand binding site and serves to dock a peptide ligand via its C-terminal segment, thus positioning the N-terminal segment to interact with the receptor's juxtamembrane domains to activate the receptor. Here we present the 3D NMR structure of ECD1 of CRF-R2beta in complex with astressin, a peptide antagonist. In the structure of the complex the C-terminal segment of astressin forms an amphipathic helix, whose entire hydrophobic face interacts with the short-consensus-repeat motif, covering a large intermolecular interface. In addition, the complex is characterized by intermolecular hydrogen bonds and a salt bridge. These interactions are quantitatively weighted by an analysis of the effects on the full-length receptor affinities using an Ala scan of CRF. These structural studies identify the major determinants for CRF ligand specificity and selectivity and support a two-step model for receptor activation. Furthermore, because of a proposed conservation of the fold for both the ECD1s and ligands, this structure can serve as a model for ligand recognition for the entire B1 receptor family. |
==About this Structure== | ==About this Structure== | ||
| - | 2JNC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 2JNC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cantle, J | + | [[Category: Cantle, J P.]] |
| - | [[Category: DiGruccio, M | + | [[Category: DiGruccio, M R.]] |
| - | [[Category: Grace, C | + | [[Category: Grace, C R.R.]] |
[[Category: Jozsef, G.]] | [[Category: Jozsef, G.]] | ||
| - | [[Category: Perrin, M | + | [[Category: Perrin, M H.]] |
[[Category: Riek, R.]] | [[Category: Riek, R.]] | ||
| - | [[Category: Rivier, J | + | [[Category: Rivier, J E.]] |
| - | [[Category: Vale, W | + | [[Category: Vale, W W.]] |
[[Category: elliptical b-sandwich]] | [[Category: elliptical b-sandwich]] | ||
[[Category: scr fold]] | [[Category: scr fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:04:23 2008'' |
Revision as of 16:04, 21 February 2008
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Refined 3D NMR structure of ECD1 of mCRF-R2beta at pH 5
Overview
The corticotropin releasing factor (CRF) family of ligands and their receptors coordinate endocrine, behavioral, autonomic, and metabolic responses to stress and play additional roles within the cardiovascular, gastrointestinal, and other systems. The actions of CRF and the related urocortins are mediated by activation of two receptors, CRF-R1 and CRF-R2, belonging to the B1 family of G protein-coupled receptors. The short-consensus-repeat fold (SCR) within the first extracellular domain (ECD1) of the CRF receptor(s) comprises the major ligand binding site and serves to dock a peptide ligand via its C-terminal segment, thus positioning the N-terminal segment to interact with the receptor's juxtamembrane domains to activate the receptor. Here we present the 3D NMR structure of ECD1 of CRF-R2beta in complex with astressin, a peptide antagonist. In the structure of the complex the C-terminal segment of astressin forms an amphipathic helix, whose entire hydrophobic face interacts with the short-consensus-repeat motif, covering a large intermolecular interface. In addition, the complex is characterized by intermolecular hydrogen bonds and a salt bridge. These interactions are quantitatively weighted by an analysis of the effects on the full-length receptor affinities using an Ala scan of CRF. These structural studies identify the major determinants for CRF ligand specificity and selectivity and support a two-step model for receptor activation. Furthermore, because of a proposed conservation of the fold for both the ECD1s and ligands, this structure can serve as a model for ligand recognition for the entire B1 receptor family.
About this Structure
2JNC is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure of the N-terminal domain of a type B1 G protein-coupled receptor in complex with a peptide ligand., Grace CR, Perrin MH, Gulyas J, Digruccio MR, Cantle JP, Rivier JE, Vale WW, Riek R, Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):4858-63. Epub 2007 Mar 12. PMID:17360332
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