2jou
From Proteopedia
(New page: 200px<br /> <applet load="2jou" size="450" color="white" frame="true" align="right" spinBox="true" caption="2jou" /> '''NMR structure of Mini-B, an N-terminal- C-t...) |
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'''NMR structure of Mini-B, an N-terminal- C-terminal construct from human Surfactant Protein-B (SP-B), in Hexafluoroisopropanol (HFIP)'''<br /> | '''NMR structure of Mini-B, an N-terminal- C-terminal construct from human Surfactant Protein-B (SP-B), in Hexafluoroisopropanol (HFIP)'''<br /> | ||
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| + | ==Overview== | ||
| + | Surfactant protein B (SP-B) is essential for normal lung surfactant function, which is in itself essential to life. However, the molecular basis for SP-B's activity is not understood and a high-resolution structure for SP-B has not been determined. Mini-B is a 34-residue peptide with internal disulfide linkages that is composed of the N- and C-terminal helical regions of SP-B. It has been shown to retain similar activity to full-length SP-B in certain in vitro and in vivo studies. We have used solution NMR to determine the structure of Mini-B in the presence of micelles composed of the anionic detergent sodium dodecyl sulfate (SDS). Under these conditions, Mini-B forms two alpha-helices connected by an unstructured loop. Mini-B possesses a strikingly amphipathic surface with a large positively charged patch on one face of the peptide and a large hydrophobic patch on the opposite face. A tryptophan side chain extends outward from the peptide in a position to interact with lipids at the polar/apolar interface. Interhelix interactions are stabilized by both disulfide bonds and by interleaving of hydrophobic side chains from the two helices. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JOU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. This structure | + | 2JOU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. This structure supersedes the now removed PDB entry 2A2H. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JOU OCA]. |
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| + | ==Reference== | ||
| + | Structure of mini-B, a functional fragment of surfactant protein B, in detergent micelles., Sarker M, Waring AJ, Walther FJ, Keough KM, Booth V, Biochemistry. 2007 Oct 2;46(39):11047-56. Epub 2007 Sep 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17845058 17845058] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Booth, V.]] | [[Category: Booth, V.]] | ||
| - | [[Category: Keough, K | + | [[Category: Keough, K M.W.]] |
[[Category: Sarker, M.]] | [[Category: Sarker, M.]] | ||
| - | [[Category: Walther, F | + | [[Category: Walther, F J.]] |
| - | [[Category: Waring, A | + | [[Category: Waring, A J.]] |
[[Category: lipid associated protein]] | [[Category: lipid associated protein]] | ||
[[Category: mini-b]] | [[Category: mini-b]] | ||
[[Category: sp-b]] | [[Category: sp-b]] | ||
| + | [[Category: surface active protein]] | ||
[[Category: surfactant protein b]] | [[Category: surfactant protein b]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:04:48 2008'' |
Revision as of 16:04, 21 February 2008
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NMR structure of Mini-B, an N-terminal- C-terminal construct from human Surfactant Protein-B (SP-B), in Hexafluoroisopropanol (HFIP)
Contents |
Overview
Surfactant protein B (SP-B) is essential for normal lung surfactant function, which is in itself essential to life. However, the molecular basis for SP-B's activity is not understood and a high-resolution structure for SP-B has not been determined. Mini-B is a 34-residue peptide with internal disulfide linkages that is composed of the N- and C-terminal helical regions of SP-B. It has been shown to retain similar activity to full-length SP-B in certain in vitro and in vivo studies. We have used solution NMR to determine the structure of Mini-B in the presence of micelles composed of the anionic detergent sodium dodecyl sulfate (SDS). Under these conditions, Mini-B forms two alpha-helices connected by an unstructured loop. Mini-B possesses a strikingly amphipathic surface with a large positively charged patch on one face of the peptide and a large hydrophobic patch on the opposite face. A tryptophan side chain extends outward from the peptide in a position to interact with lipids at the polar/apolar interface. Interhelix interactions are stabilized by both disulfide bonds and by interleaving of hydrophobic side chains from the two helices.
Disease
Known disease associated with this structure: Surfactant metabolism dysfunction, pulmonary, 1 OMIM:[178640]
About this Structure
2JOU is a Single protein structure of sequence from [1]. This structure supersedes the now removed PDB entry 2A2H. Full crystallographic information is available from OCA.
Reference
Structure of mini-B, a functional fragment of surfactant protein B, in detergent micelles., Sarker M, Waring AJ, Walther FJ, Keough KM, Booth V, Biochemistry. 2007 Oct 2;46(39):11047-56. Epub 2007 Sep 11. PMID:17845058
Page seeded by OCA on Thu Feb 21 18:04:48 2008
