1w0h

From Proteopedia

Revision as of 14:16, 30 October 2007

CRYSTALLOGRAPHIC STRUCTURE OF THE NUCLEASE DOMAIN OF 3'HEXO, A DEDDH FAMILY MEMBER, BOUND TO RAMP

Overview

A human 3'-5'-exoribonuclease (3'hExo) has recently been identified and, shown to be responsible for histone mRNA degradation. Functionally, 3'hExo, and a stem-loop binding protein (SLBP) target opposite faces of a unique, highly conserved stem-loop RNA scaffold towards the 3' end of histone, mRNA, which is composed of a 6 bp stem and a 4 nt loop, followed by an, ACCCA sequence. Its Caenorhabditis elegans homologue, ERI-1, has been, shown to degrade small interfering RNA in vitro and to function as a, negative regulator of RNA interference in neuronal cells. We have, determined the structure of the nuclease domain (Nuc) of 3'hExo complexed, with rAMP in the presence of Mg2+ at 1.6 A resolution. The Nuc domain, adopts an alpha/beta globular fold, with four acidic residues coordinating, a ... [(full description)]

About this Structure

1W0H is a [Single protein] structure of sequence from [Homo sapiens] with MG and AMP as [ligands]. Structure known Active Site: MGA. Full crystallographic information is available from [OCA].

Reference

Crystallographic structure of the nuclease domain of 3'hExo, a DEDDh family member, bound to rAMP., Cheng Y, Patel DJ, J Mol Biol. 2004 Oct 15;343(2):305-12. PMID:15451662

Page seeded by OCA on Tue Oct 30 16:20:58 2007