2kau
From Proteopedia
(New page: 200px<br /><applet load="2kau" size="450" color="white" frame="true" align="right" spinBox="true" caption="2kau, resolution 2.0Å" /> '''THE CRYSTAL STRUCTURE...) |
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| - | [[Image:2kau.jpg|left|200px]]<br /><applet load="2kau" size=" | + | [[Image:2kau.jpg|left|200px]]<br /><applet load="2kau" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2kau, resolution 2.0Å" /> | caption="2kau, resolution 2.0Å" /> | ||
'''THE CRYSTAL STRUCTURE OF UREASE FROM KLEBSIELLA AEROGENES AT 2.2 ANGSTROMS RESOLUTION'''<br /> | '''THE CRYSTAL STRUCTURE OF UREASE FROM KLEBSIELLA AEROGENES AT 2.2 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of urease from Klebsiella aerogenes has been | + | The crystal structure of urease from Klebsiella aerogenes has been determined at 2.2 A resolution and refined to an R factor of 18.2 percent. The enzyme contains four structural domains: three with novel folds playing structural roles, and an (alpha beta)8 barrel domain, which contains the bi-nickel center. The two active site nickels are 3.5 A apart. One nickel ion is coordinated by three ligands (with low occupancy of a fourth ligand) and the second is coordinated by five ligands. A carbamylated lysine provides an oxygen ligand to each nickel, explaining why carbon dioxide is required for the activation of urease apoenzyme. The structure is compatible with a catalytic mechanism whereby urea ligates Ni-1 to complete its tetrahedral coordination and a hydroxide ligand of Ni-2 attacks the carbonyl carbon. A surprisingly high structural similarity between the urease catalytic domain and that of the zinc-dependent adenosine deaminase reveals a remarkable example of active site divergence. |
==About this Structure== | ==About this Structure== | ||
| - | 2KAU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes] with NI and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] Full crystallographic information is available from [http:// | + | 2KAU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes] with <scene name='pdbligand=NI:'>NI</scene> and <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Urease]] | [[Category: Urease]] | ||
| - | [[Category: Carr, M | + | [[Category: Carr, M B.]] |
| - | [[Category: Hausinger, R | + | [[Category: Hausinger, R P.]] |
[[Category: Jabri, E.]] | [[Category: Jabri, E.]] | ||
| - | [[Category: Karplus, P | + | [[Category: Karplus, P A.]] |
[[Category: FMT]] | [[Category: FMT]] | ||
[[Category: NI]] | [[Category: NI]] | ||
[[Category: nickel metalloenzyme]] | [[Category: nickel metalloenzyme]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:06:50 2008'' |
Revision as of 16:06, 21 February 2008
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THE CRYSTAL STRUCTURE OF UREASE FROM KLEBSIELLA AEROGENES AT 2.2 ANGSTROMS RESOLUTION
Overview
The crystal structure of urease from Klebsiella aerogenes has been determined at 2.2 A resolution and refined to an R factor of 18.2 percent. The enzyme contains four structural domains: three with novel folds playing structural roles, and an (alpha beta)8 barrel domain, which contains the bi-nickel center. The two active site nickels are 3.5 A apart. One nickel ion is coordinated by three ligands (with low occupancy of a fourth ligand) and the second is coordinated by five ligands. A carbamylated lysine provides an oxygen ligand to each nickel, explaining why carbon dioxide is required for the activation of urease apoenzyme. The structure is compatible with a catalytic mechanism whereby urea ligates Ni-1 to complete its tetrahedral coordination and a hydroxide ligand of Ni-2 attacks the carbonyl carbon. A surprisingly high structural similarity between the urease catalytic domain and that of the zinc-dependent adenosine deaminase reveals a remarkable example of active site divergence.
About this Structure
2KAU is a Protein complex structure of sequences from Klebsiella aerogenes with and as ligands. Active as Urease, with EC number 3.5.1.5 Full crystallographic information is available from OCA.
Reference
The crystal structure of urease from Klebsiella aerogenes., Jabri E, Carr MB, Hausinger RP, Karplus PA, Science. 1995 May 19;268(5213):998-1004. PMID:7754395
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