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2knt
From Proteopedia
(New page: 200px<br /> <applet load="2knt" size="450" color="white" frame="true" align="right" spinBox="true" caption="2knt, resolution 1.20Å" /> '''THE 1.2 ANGSTROM ST...) |
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| - | [[Image:2knt.gif|left|200px]]<br /> | + | [[Image:2knt.gif|left|200px]]<br /><applet load="2knt" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2knt" size=" | + | |
caption="2knt, resolution 1.20Å" /> | caption="2knt, resolution 1.20Å" /> | ||
'''THE 1.2 ANGSTROM STRUCTURE OF KUNITZ TYPE DOMAIN C5'''<br /> | '''THE 1.2 ANGSTROM STRUCTURE OF KUNITZ TYPE DOMAIN C5'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The recombinant Kunitz-type domain (C5) of human collagen alpha3(VI) chain | + | The recombinant Kunitz-type domain (C5) of human collagen alpha3(VI) chain was previously described at 1.6 A resolution at room temperature. By changing the crystallization conditions and using synchrotron radiation, we are able to record diffraction data to 1.2 A resolution for crystals of the same space group at 291 K. The protein-water-ion model has been refined anisotropically against these new data using the program SHELXL93; the results converged to an R factor of 15.0%, with all data between 7 and 1.2 A. The final electron-density map reveals a clear chain tracing with a few disordered residues and five residues out of 58 that present alternate conformations. The Cys14-Cys38 bond presents the less frequently observed left-hand conformation (chi1 = -60 degrees). The solvent molecules and a phosphate ion are well ordered with an average B of 38 A2. The high-resolution structure reveals the N and C termini which were missing from the 1.6 A structure. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2KNT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2KNT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KNT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: kunitz inhibitor]] | [[Category: kunitz inhibitor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:06:53 2008'' |
Revision as of 16:06, 21 February 2008
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THE 1.2 ANGSTROM STRUCTURE OF KUNITZ TYPE DOMAIN C5
Contents |
Overview
The recombinant Kunitz-type domain (C5) of human collagen alpha3(VI) chain was previously described at 1.6 A resolution at room temperature. By changing the crystallization conditions and using synchrotron radiation, we are able to record diffraction data to 1.2 A resolution for crystals of the same space group at 291 K. The protein-water-ion model has been refined anisotropically against these new data using the program SHELXL93; the results converged to an R factor of 15.0%, with all data between 7 and 1.2 A. The final electron-density map reveals a clear chain tracing with a few disordered residues and five residues out of 58 that present alternate conformations. The Cys14-Cys38 bond presents the less frequently observed left-hand conformation (chi1 = -60 degrees). The solvent molecules and a phosphate ion are well ordered with an average B of 38 A2. The high-resolution structure reveals the N and C termini which were missing from the 1.6 A structure.
Disease
Known diseases associated with this structure: Bethlem myopathy OMIM:[120250], Ullrich congenital muscular dystrophy OMIM:[120250]
About this Structure
2KNT is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
1.2 A refinement of the Kunitz-type domain from the alpha3 chain of human type VI collagen., Merigeau K, Arnoux B, Perahia D, Norris K, Norris F, Ducruix A, Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):306-12. PMID:9761897
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