2nmb
From Proteopedia
(New page: 200px<br /><applet load="2nmb" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nmb" /> '''DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYR...) |
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| - | [[Image:2nmb.jpg|left|200px]]<br /><applet load="2nmb" size=" | + | [[Image:2nmb.jpg|left|200px]]<br /><applet load="2nmb" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2nmb" /> | caption="2nmb" /> | ||
'''DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.'''<br /> | '''DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The phosphotyrosine-binding (PTB) domain of Numb, a protein involved in | + | The phosphotyrosine-binding (PTB) domain of Numb, a protein involved in asymmetric cell division, has recently been shown to bind to the adapter protein Lnx through an LDNPAY sequence, to the Numb-associated kinase (Nak) through a sequence that does not contain an NPXY motif and to GP(p)Y-containing peptides obtained from library screening. We show here that these diverse peptide sequences bind with comparable affinities to the Numb PTB domain at a common binding site on the surface of the protein. The NMR structure of the Numb PTB domain in complex with a GPpY-containing peptide reveals a novel mechanism of binding with the peptide in a helical turn that does not hydrogen bond to the PTB domain beta-sheet. These results suggest that PTB domains can potentially have multiple modes of peptide recognition and provide a structural basis from which the multiple functions of the Numb PTB domain during asymmetric cell division could arise. |
==About this Structure== | ==About this Structure== | ||
| - | 2NMB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 2NMB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NMB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Forman-Kay, J | + | [[Category: Forman-Kay, J D.]] |
| - | [[Category: Li, S | + | [[Category: Li, S C.]] |
| - | [[Category: McGlade, C | + | [[Category: McGlade, C J.]] |
[[Category: Pawson,T.]] | [[Category: Pawson,T.]] | ||
| - | [[Category: Vincent, S | + | [[Category: Vincent, S J.F.]] |
[[Category: Zwahlen, C.]] | [[Category: Zwahlen, C.]] | ||
[[Category: asymetr ic cell division]] | [[Category: asymetr ic cell division]] | ||
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[[Category: signal transduction]] | [[Category: signal transduction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:08:26 2008'' |
Revision as of 16:08, 21 February 2008
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DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.
Overview
The phosphotyrosine-binding (PTB) domain of Numb, a protein involved in asymmetric cell division, has recently been shown to bind to the adapter protein Lnx through an LDNPAY sequence, to the Numb-associated kinase (Nak) through a sequence that does not contain an NPXY motif and to GP(p)Y-containing peptides obtained from library screening. We show here that these diverse peptide sequences bind with comparable affinities to the Numb PTB domain at a common binding site on the surface of the protein. The NMR structure of the Numb PTB domain in complex with a GPpY-containing peptide reveals a novel mechanism of binding with the peptide in a helical turn that does not hydrogen bond to the PTB domain beta-sheet. These results suggest that PTB domains can potentially have multiple modes of peptide recognition and provide a structural basis from which the multiple functions of the Numb PTB domain during asymmetric cell division could arise.
About this Structure
2NMB is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structure of a Numb PTB domain-peptide complex suggests a basis for diverse binding specificity., Li SC, Zwahlen C, Vincent SJ, McGlade CJ, Kay LE, Pawson T, Forman-Kay JD, Nat Struct Biol. 1998 Dec;5(12):1075-83. PMID:9846878
Page seeded by OCA on Thu Feb 21 18:08:26 2008
