2nnz
From Proteopedia
(New page: 200px<br /><applet load="2nnz" size="350" color="white" frame="true" align="right" spinBox="true" caption="2nnz" /> '''Solution structure of the hypothetical prote...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | AF2241 is a hypothetical protein from Archaeoglobus fulgidus and it | + | AF2241 is a hypothetical protein from Archaeoglobus fulgidus and it belongs to the PFam domain of unknown function 369 (DUF369). NMR structural determination reveals that AF2241 adopts a cyclophilin-like fold, with a beta-barrel core composed of eight beta-strands, one alpha-helix, and one 3(10) helix located at each end of the barrel. The protein displays a high structural similarity to TM1367, another member of DUF369 whose structure has been determined recently by X-ray crystallography. Structural similarity search shows that AF2241 also has a high similarity to human cyclophilin A, however, sequence alignment and electrostatic potential analysis reveal that the residues in the PPIase catalytic site of human cyclophilin A are not conserved in AF2241 or TM1367. Instead, a putative active site of AF2241 maps to a negatively charged pocket composed of 9 conserved residues. Our results suggest that although AF2241 adopts the same fold as the human cyclophilin A, it may have distinct biological function. |
==About this Structure== | ==About this Structure== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ai, X.]] | [[Category: Ai, X.]] | ||
| - | [[Category: Arrowsmith, C | + | [[Category: Arrowsmith, C H.]] |
| - | [[Category: Choy, W | + | [[Category: Choy, W Y.]] |
| - | [[Category: Li, S | + | [[Category: Li, S S.C.]] |
| - | [[Category: OCSP, Ontario | + | [[Category: OCSP, Ontario Centre for Structural Proteomics.]] |
[[Category: Semesib, A.]] | [[Category: Semesib, A.]] | ||
[[Category: Yee, A.]] | [[Category: Yee, A.]] | ||
| Line 26: | Line 26: | ||
[[Category: unknown function]] | [[Category: unknown function]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:08:53 2008'' |
Revision as of 16:08, 21 February 2008
|
Solution structure of the hypothetical protein AF2241 from Archaeoglobus fulgidus
Overview
AF2241 is a hypothetical protein from Archaeoglobus fulgidus and it belongs to the PFam domain of unknown function 369 (DUF369). NMR structural determination reveals that AF2241 adopts a cyclophilin-like fold, with a beta-barrel core composed of eight beta-strands, one alpha-helix, and one 3(10) helix located at each end of the barrel. The protein displays a high structural similarity to TM1367, another member of DUF369 whose structure has been determined recently by X-ray crystallography. Structural similarity search shows that AF2241 also has a high similarity to human cyclophilin A, however, sequence alignment and electrostatic potential analysis reveal that the residues in the PPIase catalytic site of human cyclophilin A are not conserved in AF2241 or TM1367. Instead, a putative active site of AF2241 maps to a negatively charged pocket composed of 9 conserved residues. Our results suggest that although AF2241 adopts the same fold as the human cyclophilin A, it may have distinct biological function.
About this Structure
2NNZ is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Hypothetical protein AF2241 from Archaeoglobus fulgidus adopts a cyclophilin-like fold., Ai X, Li L, Semesi A, Yee A, Arrowsmith CH, Li SS, Choy WY, J Biomol NMR. 2007 Aug;38(4):353-8. Epub 2007 Jul 4. PMID:17610131
Page seeded by OCA on Thu Feb 21 18:08:53 2008
