2nos
From Proteopedia
(New page: 200px<br /><applet load="2nos" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nos, resolution 2.3Å" /> '''MURINE INDUCIBLE NITR...) |
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| - | [[Image:2nos.jpg|left|200px]]<br /><applet load="2nos" size=" | + | [[Image:2nos.jpg|left|200px]]<br /><applet load="2nos" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2nos, resolution 2.3Å" /> | caption="2nos, resolution 2.3Å" /> | ||
'''MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114), AMINOGUANIDINE COMPLEX'''<br /> | '''MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114), AMINOGUANIDINE COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The nitric oxide synthase oxygenase domain (NOSox) oxidizes arginine to | + | The nitric oxide synthase oxygenase domain (NOSox) oxidizes arginine to synthesize the cellular signal and defensive cytotoxin nitric oxide (NO). Crystal structures determined for cytokine-inducible NOSox reveal an unusual fold and heme environment for stabilization of activated oxygen intermediates key for catalysis. A winged beta sheet engenders a curved alpha-beta domain resembling a baseball catcher's mitt with heme clasped in the palm. The location of exposed hydrophobic residues and the results of mutational analysis place the dimer interface adjacent to the heme-binding pocket. Juxtaposed hydrophobic O2- and polar L-arginine-binding sites occupied by imidazole and aminoguanidine, respectively, provide a template for designing dual-function inhibitors and imply substrate-assisted catalysis. |
==About this Structure== | ==About this Structure== | ||
| - | 2NOS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with HEM, IMD and AGU as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http:// | + | 2NOS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=IMD:'>IMD</scene> and <scene name='pdbligand=AGU:'>AGU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NOS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Nitric-oxide synthase]] | [[Category: Nitric-oxide synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arvai, A | + | [[Category: Arvai, A S.]] |
| - | [[Category: Crane, B | + | [[Category: Crane, B R.]] |
| - | [[Category: Getzoff, E | + | [[Category: Getzoff, E D.]] |
| - | [[Category: Stuehr, D | + | [[Category: Stuehr, D J.]] |
| - | [[Category: Tainer, J | + | [[Category: Tainer, J A.]] |
[[Category: AGU]] | [[Category: AGU]] | ||
[[Category: HEM]] | [[Category: HEM]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:09:13 2008'' |
Revision as of 16:09, 21 February 2008
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MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114), AMINOGUANIDINE COMPLEX
Overview
The nitric oxide synthase oxygenase domain (NOSox) oxidizes arginine to synthesize the cellular signal and defensive cytotoxin nitric oxide (NO). Crystal structures determined for cytokine-inducible NOSox reveal an unusual fold and heme environment for stabilization of activated oxygen intermediates key for catalysis. A winged beta sheet engenders a curved alpha-beta domain resembling a baseball catcher's mitt with heme clasped in the palm. The location of exposed hydrophobic residues and the results of mutational analysis place the dimer interface adjacent to the heme-binding pocket. Juxtaposed hydrophobic O2- and polar L-arginine-binding sites occupied by imidazole and aminoguanidine, respectively, provide a template for designing dual-function inhibitors and imply substrate-assisted catalysis.
About this Structure
2NOS is a Single protein structure of sequence from Mus musculus with , and as ligands. Active as Nitric-oxide synthase, with EC number 1.14.13.39 Full crystallographic information is available from OCA.
Reference
The structure of nitric oxide synthase oxygenase domain and inhibitor complexes., Crane BR, Arvai AS, Gachhui R, Wu C, Ghosh DK, Getzoff ED, Stuehr DJ, Tainer JA, Science. 1997 Oct 17;278(5337):425-31. PMID:9334294
Page seeded by OCA on Thu Feb 21 18:09:13 2008
Categories: Mus musculus | Nitric-oxide synthase | Single protein | Arvai, A S. | Crane, B R. | Getzoff, E D. | Stuehr, D J. | Tainer, J A. | AGU | HEM | IMD | Aminoguanidine | Heme | L-arginine monooxygenase | Nitric oxide | No | Nos | Oxidoreductase
