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2nq3

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(New page: 200px<br /> <applet load="2nq3" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nq3, resolution 1.80&Aring;" /> '''Crystal structure o...)
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'''Crystal structure of the C2 Domain of Human Itchy Homolog E3 Ubiquitin Protein Ligase'''<br />
'''Crystal structure of the C2 Domain of Human Itchy Homolog E3 Ubiquitin Protein Ligase'''<br />
==Overview==
==Overview==
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We have cloned a new protein that interacts with the hematopoietic, DNA-binding transcription factor, p45/NF-E2, by screening a human, erythroleukemia cell cDNA library with the yeast two-hybrid approach., Predicted peptide sequence and chromosomal mapping identified the cloned, molecule to be the product of the human ortholog of the mouse Itch gene, which has been implicated previously in the regulation of growth and, differentiation of erythroid and lymphoid cells. Transfection experiments, indicate that this human ITCH protein can act as a transcriptional, corepressor of p45/NF-E2. Our data provide novel insights into the, functional roles of the mammalian ITCH proteins in the development of, hematopoietic cell lineages.
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We have cloned a new protein that interacts with the hematopoietic DNA-binding transcription factor, p45/NF-E2, by screening a human erythroleukemia cell cDNA library with the yeast two-hybrid approach. Predicted peptide sequence and chromosomal mapping identified the cloned molecule to be the product of the human ortholog of the mouse Itch gene, which has been implicated previously in the regulation of growth and differentiation of erythroid and lymphoid cells. Transfection experiments indicate that this human ITCH protein can act as a transcriptional corepressor of p45/NF-E2. Our data provide novel insights into the functional roles of the mammalian ITCH proteins in the development of hematopoietic cell lineages.
==About this Structure==
==About this Structure==
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2NQ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2NQ3 OCA].
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2NQ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NQ3 OCA].
==Reference==
==Reference==
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Arrowsmith, C.H.]]
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[[Category: Arrowsmith, C H.]]
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[[Category: Avvakumov, G.V.]]
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[[Category: Avvakumov, G V.]]
[[Category: Bochkarev, A.]]
[[Category: Bochkarev, A.]]
[[Category: Butler-Cole, C.]]
[[Category: Butler-Cole, C.]]
[[Category: Dhe-Paganon, S.]]
[[Category: Dhe-Paganon, S.]]
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[[Category: Edwards, A.M.]]
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[[Category: Edwards, A M.]]
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[[Category: Jr., P.J.Finerty.]]
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[[Category: Jr., P J.Finerty.]]
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[[Category: SGC, Structural.Genomics.Consortium.]]
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[[Category: SGC, Structural Genomics Consortium.]]
[[Category: Sundstrom, M.]]
[[Category: Sundstrom, M.]]
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[[Category: Walker, J.R.]]
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[[Category: Walker, J R.]]
[[Category: Weigelt, J.]]
[[Category: Weigelt, J.]]
[[Category: Xue, S.]]
[[Category: Xue, S.]]
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[[Category: ubl conjugation pathway]]
[[Category: ubl conjugation pathway]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:01:42 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:09:39 2008''

Revision as of 16:09, 21 February 2008


2nq3, resolution 1.80Å

Drag the structure with the mouse to rotate

Crystal structure of the C2 Domain of Human Itchy Homolog E3 Ubiquitin Protein Ligase

Overview

We have cloned a new protein that interacts with the hematopoietic DNA-binding transcription factor, p45/NF-E2, by screening a human erythroleukemia cell cDNA library with the yeast two-hybrid approach. Predicted peptide sequence and chromosomal mapping identified the cloned molecule to be the product of the human ortholog of the mouse Itch gene, which has been implicated previously in the regulation of growth and differentiation of erythroid and lymphoid cells. Transfection experiments indicate that this human ITCH protein can act as a transcriptional corepressor of p45/NF-E2. Our data provide novel insights into the functional roles of the mammalian ITCH proteins in the development of hematopoietic cell lineages.

About this Structure

2NQ3 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.

Reference

Human ITCH is a coregulator of the hematopoietic transcription factor NF-E2., Chen X, Wen S, Fukuda MN, Gavva NR, Hsu D, Akama TO, Yang-Feng T, Shen CK, Genomics. 2001 Apr 15;73(2):238-41. PMID:11318614

Page seeded by OCA on Thu Feb 21 18:09:39 2008

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