2nuo
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) | + | Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Wlodawer, A.]] | [[Category: Wlodawer, A.]] | ||
| - | [[Category: Ziolkowska, N | + | [[Category: Ziolkowska, N E.]] |
[[Category: EDO]] | [[Category: EDO]] | ||
[[Category: GLC]] | [[Category: GLC]] | ||
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[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:11:16 2008'' |
Revision as of 16:11, 21 February 2008
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Crystal structure of a complex of griffithsin with glucose
Overview
Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes.
About this Structure
2NUO is a Single protein structure of sequence from Griffithsia with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736
Page seeded by OCA on Thu Feb 21 18:11:16 2008
Categories: Griffithsia | Single protein | Wlodawer, A. | Ziolkowska, N E. | EDO | GLC | SO4 | Domain swapping | Griffithsin | Hiv | Lectins | Mannose binding | Sars | Sugar binding protein
