2nz4
From Proteopedia
(New page: 200px<br /> <applet load="2nz4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nz4, resolution 2.498Å" /> '''Structural investi...) |
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| - | [[Image:2nz4.gif|left|200px]]<br /> | + | [[Image:2nz4.gif|left|200px]]<br /><applet load="2nz4" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2nz4" size=" | + | |
caption="2nz4, resolution 2.498Å" /> | caption="2nz4, resolution 2.498Å" /> | ||
'''Structural investigation of the GlmS ribozyme bound to its catalytic cofactor'''<br /> | '''Structural investigation of the GlmS ribozyme bound to its catalytic cofactor'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The GlmS riboswitch is located in the 5'-untranslated region of the gene | + | The GlmS riboswitch is located in the 5'-untranslated region of the gene encoding glucosamine-6-phosphate (GlcN6P) synthetase. The GlmS riboswitch is a ribozyme with activity triggered by binding of the metabolite GlcN6P. Presented here is the structure of the GlmS ribozyme (2.5 A resolution) with GlcN6P bound in the active site. The GlmS ribozyme adopts a compact double pseudoknot tertiary structure, with two closely packed helical stacks. Recognition of GlcN6P is achieved through coordination of the phosphate moiety by two hydrated magnesium ions as well as specific nucleobase contacts to the GlcN6P sugar ring. Comparison of this activator bound and the previously published apoenzyme complex supports a model in which GlcN6P does not induce a conformational change in the RNA, as is typical of other riboswitches, but instead functions as a catalytic cofactor for the reaction. This demonstrates that RNA, like protein enzymes, can employ the chemical diversity of small molecules to promote catalytic activity. |
==About this Structure== | ==About this Structure== | ||
| - | 2NZ4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GLP and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2NZ4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GLP:'>GLP</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZ4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cochrane, J | + | [[Category: Cochrane, J C.]] |
[[Category: GLP]] | [[Category: GLP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: structural protein/rna]] | [[Category: structural protein/rna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:12:40 2008'' |
Revision as of 16:12, 21 February 2008
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Structural investigation of the GlmS ribozyme bound to its catalytic cofactor
Overview
The GlmS riboswitch is located in the 5'-untranslated region of the gene encoding glucosamine-6-phosphate (GlcN6P) synthetase. The GlmS riboswitch is a ribozyme with activity triggered by binding of the metabolite GlcN6P. Presented here is the structure of the GlmS ribozyme (2.5 A resolution) with GlcN6P bound in the active site. The GlmS ribozyme adopts a compact double pseudoknot tertiary structure, with two closely packed helical stacks. Recognition of GlcN6P is achieved through coordination of the phosphate moiety by two hydrated magnesium ions as well as specific nucleobase contacts to the GlcN6P sugar ring. Comparison of this activator bound and the previously published apoenzyme complex supports a model in which GlcN6P does not induce a conformational change in the RNA, as is typical of other riboswitches, but instead functions as a catalytic cofactor for the reaction. This demonstrates that RNA, like protein enzymes, can employ the chemical diversity of small molecules to promote catalytic activity.
About this Structure
2NZ4 is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Structural investigation of the GlmS ribozyme bound to Its catalytic cofactor., Cochrane JC, Lipchock SV, Strobel SA, Chem Biol. 2007 Jan;14(1):97-105. Epub 2006 Dec 28. PMID:17196404
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