1w36

From Proteopedia

Revision as of 14:18, 30 October 2007

RECBCD:DNA COMPLEX

Overview

RecBCD is a multi-functional enzyme complex that processes DNA ends, resulting from a double-strand break. RecBCD is a bipolar helicase that, splits the duplex into its component strands and digests them until, encountering a recombinational hotspot (Chi site). The nuclease activity, is then attenuated and RecBCD loads RecA onto the 3' tail of the DNA. Here, we present the crystal structure of RecBCD bound to a DNA substrate. In, this initiation complex, the DNA duplex has been split across the RecC, subunit to create a fork with the separated strands each heading towards, different helicase motor subunits. The strands pass along tunnels within, the complex, both emerging adjacent to the nuclease domain of RecB., Passage of the 3' tail through one of these tunnels provides a mechanism, ... [(full description)]

About this Structure

1W36 is a [Protein complex] structure of sequences from [Escherichia coli] with CA as [ligand]. Active as [Exodeoxyribonuclease V], with EC number [3.1.11.5]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks., Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB, Nature. 2004 Nov 11;432(7014):187-93. PMID:15538360

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