2o3w

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(New page: 200px<br /><applet load="2o3w" size="350" color="white" frame="true" align="right" spinBox="true" caption="2o3w, resolution 2.800&Aring;" /> '''Crystal Structure o...)
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==Overview==
==Overview==
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The lack of absolute prokaryotic selectivity of natural antibiotics is, widespread and is a significant clinical problem. The use of this, disadvantage of aminoglycoside antibiotics for the possible treatment of, human genetic diseases is extremely challenging. Here, we have used a, combination of biochemical and structural analysis to compare and contrast, the molecular mechanisms of action and the structure-activity, relationships of a new synthetic aminoglycoside, NB33, and a structurally, similar natural aminoglycoside apramycin. The data presented herein, demonstrate the general molecular principles that determine the decreased, selectivity of apramycin for the prokaryotic decoding site, and the, increased selectivity of NB33 for the eukaryotic decoding site. These, results are therefore extremely beneficial for further research on both, the design of new aminoglycoside-based antibiotics with diminished, deleterious effects on humans, as well as the design of new, aminoglycoside-based structures that selectively target the eukaryotic, ribosome.
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The lack of absolute prokaryotic selectivity of natural antibiotics is widespread and is a significant clinical problem. The use of this disadvantage of aminoglycoside antibiotics for the possible treatment of human genetic diseases is extremely challenging. Here, we have used a combination of biochemical and structural analysis to compare and contrast the molecular mechanisms of action and the structure-activity relationships of a new synthetic aminoglycoside, NB33, and a structurally similar natural aminoglycoside apramycin. The data presented herein demonstrate the general molecular principles that determine the decreased selectivity of apramycin for the prokaryotic decoding site, and the increased selectivity of NB33 for the eukaryotic decoding site. These results are therefore extremely beneficial for further research on both the design of new aminoglycoside-based antibiotics with diminished deleterious effects on humans, as well as the design of new aminoglycoside-based structures that selectively target the eukaryotic ribosome.
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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Differential Selectivity of Natural and Synthetic Aminoglycosides towards the Eukaryotic and Prokaryotic Decoding A Sites., Kondo J, Hainrichson M, Nudelman I, Shallom-Shezifi D, Barbieri CM, Pilch DS, Westhof E, Baasov T, Chembiochem. 2007 Sep 24;8(14):1700-1709. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17705310 17705310]
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Differential selectivity of natural and synthetic aminoglycosides towards the eukaryotic and prokaryotic decoding A sites., Kondo J, Hainrichson M, Nudelman I, Shallom-Shezifi D, Barbieri CM, Pilch DS, Westhof E, Baasov T, Chembiochem. 2007 Sep 24;8(14):1700-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17705310 17705310]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Baasov, T.]]
[[Category: Baasov, T.]]
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[[Category: unspecific binding]]
[[Category: unspecific binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:21:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:14:09 2008''

Revision as of 16:14, 21 February 2008

Image:2o3w.gif

2o3w, resolution 2.800Å

Drag the structure with the mouse to rotate

Crystal Structure of the Homo sapiens Cytoplasmic Ribosomal Decoding Site in presence of paromomycin

Overview

The lack of absolute prokaryotic selectivity of natural antibiotics is widespread and is a significant clinical problem. The use of this disadvantage of aminoglycoside antibiotics for the possible treatment of human genetic diseases is extremely challenging. Here, we have used a combination of biochemical and structural analysis to compare and contrast the molecular mechanisms of action and the structure-activity relationships of a new synthetic aminoglycoside, NB33, and a structurally similar natural aminoglycoside apramycin. The data presented herein demonstrate the general molecular principles that determine the decreased selectivity of apramycin for the prokaryotic decoding site, and the increased selectivity of NB33 for the eukaryotic decoding site. These results are therefore extremely beneficial for further research on both the design of new aminoglycoside-based antibiotics with diminished deleterious effects on humans, as well as the design of new aminoglycoside-based structures that selectively target the eukaryotic ribosome.

About this Structure

2O3W is a Single protein structure of sequence from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Differential selectivity of natural and synthetic aminoglycosides towards the eukaryotic and prokaryotic decoding A sites., Kondo J, Hainrichson M, Nudelman I, Shallom-Shezifi D, Barbieri CM, Pilch DS, Westhof E, Baasov T, Chembiochem. 2007 Sep 24;8(14):1700-9. PMID:17705310

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