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2o5n
From Proteopedia
(New page: 200px<br /><applet load="2o5n" size="350" color="white" frame="true" align="right" spinBox="true" caption="2o5n, resolution 2.400Å" /> '''Crystal structure o...) |
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==Overview== | ==Overview== | ||
| - | Mouse cytomegalovirus (MCMV), a | + | Mouse cytomegalovirus (MCMV), a beta-herpesvirus that establishes latent and persistent infections in mice, is a valuable model for studying complex virus-host interactions. MCMV encodes the m145 family of putative immunoevasins with predicted major histocompatibility complex, class I (MHC-I) structure. Functions attributed to some family members include down-regulation of host MHC-I (m152) and NKG2D ligands (m145, m152, and m155) and interaction with inhibitory or activating NK receptors (m157). We present the cellular, biochemical, and structural characterization of m153, which is a heavily glycosylated homodimer, that does not require beta2m or peptide and is expressed at the surface of MCMV-infected cells. Its 2.4-A crystal structure confirms that this compact molecule preserves an MHC-I-like fold and reveals a novel mode of dimerization, confirmed by site-directed mutagenesis, and a distinctive disulfide-stabilized extended N terminus. The structure provides a useful framework for comparative analysis of the divergent members of the m145 family. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Cellular expression and crystal structure of the murine cytomegalovirus | + | Cellular expression and crystal structure of the murine cytomegalovirus major histocompatibility complex class I-like glycoprotein, m153., Mans J, Natarajan K, Balbo A, Schuck P, Eikel D, Hess S, Robinson H, Simic H, Jonjic S, Tiemessen CT, Margulies DH, J Biol Chem. 2007 Nov 30;282(48):35247-58. Epub 2007 Sep 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17897947 17897947] |
[[Category: Murid herpesvirus 1]] | [[Category: Murid herpesvirus 1]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Mans, J.]] | [[Category: Mans, J.]] | ||
| - | [[Category: Margulies, D | + | [[Category: Margulies, D H.]] |
[[Category: Natarajan, K.]] | [[Category: Natarajan, K.]] | ||
[[Category: Robinson, H.]] | [[Category: Robinson, H.]] | ||
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[[Category: viral protein]] | [[Category: viral protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:14:43 2008'' |
Revision as of 16:14, 21 February 2008
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Crystal structure of a Viral Glycoprotein
Overview
Mouse cytomegalovirus (MCMV), a beta-herpesvirus that establishes latent and persistent infections in mice, is a valuable model for studying complex virus-host interactions. MCMV encodes the m145 family of putative immunoevasins with predicted major histocompatibility complex, class I (MHC-I) structure. Functions attributed to some family members include down-regulation of host MHC-I (m152) and NKG2D ligands (m145, m152, and m155) and interaction with inhibitory or activating NK receptors (m157). We present the cellular, biochemical, and structural characterization of m153, which is a heavily glycosylated homodimer, that does not require beta2m or peptide and is expressed at the surface of MCMV-infected cells. Its 2.4-A crystal structure confirms that this compact molecule preserves an MHC-I-like fold and reveals a novel mode of dimerization, confirmed by site-directed mutagenesis, and a distinctive disulfide-stabilized extended N terminus. The structure provides a useful framework for comparative analysis of the divergent members of the m145 family.
About this Structure
2O5N is a Protein complex structure of sequences from Murid herpesvirus 1 with as ligand. Full crystallographic information is available from OCA.
Reference
Cellular expression and crystal structure of the murine cytomegalovirus major histocompatibility complex class I-like glycoprotein, m153., Mans J, Natarajan K, Balbo A, Schuck P, Eikel D, Hess S, Robinson H, Simic H, Jonjic S, Tiemessen CT, Margulies DH, J Biol Chem. 2007 Nov 30;282(48):35247-58. Epub 2007 Sep 26. PMID:17897947
Page seeded by OCA on Thu Feb 21 18:14:43 2008
