This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2o9l
From Proteopedia
(New page: 200px<br /><applet load="2o9l" size="350" color="white" frame="true" align="right" spinBox="true" caption="2o9l" /> '''AMBER refined NMR Structure of the Sigma-54 ...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene | + | The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP. |
==About this Structure== | ==About this Structure== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Doucleff, M.]] | [[Category: Doucleff, M.]] | ||
| - | [[Category: Lee, P | + | [[Category: Lee, P S.]] |
| - | [[Category: Pelton, J | + | [[Category: Pelton, J G.]] |
| - | [[Category: Wemmer, D | + | [[Category: Wemmer, D E.]] |
[[Category: amber]] | [[Category: amber]] | ||
[[Category: generalized born solvent model]] | [[Category: generalized born solvent model]] | ||
| Line 25: | Line 25: | ||
[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:16:08 2008'' |
Revision as of 16:16, 21 February 2008
|
AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element
Overview
The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP.
About this Structure
2O9L is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Structural basis of DNA recognition by the alternative sigma-factor, sigma54., Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE, J Mol Biol. 2007 Jun 15;369(4):1070-8. Epub 2007 Apr 12. PMID:17481658
Page seeded by OCA on Thu Feb 21 18:16:08 2008
