2obl
From Proteopedia
(New page: 200px<br /><applet load="2obl" size="350" color="white" frame="true" align="right" spinBox="true" caption="2obl, resolution 1.80Å" /> '''Structural and bioch...) |
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==Overview== | ==Overview== | ||
| - | The type III secretion system (T3SS) ATPase is the conserved and essential | + | The type III secretion system (T3SS) ATPase is the conserved and essential inner-membrane component involved in the initial stages of selective secretion of specialized T3SS virulence effector proteins from the bacterial cytoplasm through to the infected host cell, a process crucial to subsequent pathogenicity. Here we present the 1.8-A-resolution crystal structure of the catalytic domain of the prototypical T3SS ATPase EscN from enteropathogenic Escherichia coli (EPEC). Along with in vitro and in vivo mutational analysis, our data show that the T3SS ATPases share similarity with the F1 ATPases but have important structural and sequence differences that dictate their unique secretory role. We also show that T3SS ATPase activity is dependent on EscN oligomerization and describe the molecular features and possible functional implications of a hexameric ring model. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Deng, W.]] | [[Category: Deng, W.]] | ||
| - | [[Category: Finlay, B | + | [[Category: Finlay, B B.]] |
| - | [[Category: Strynadka, N | + | [[Category: Strynadka, N C.J.]] |
[[Category: Vuckovic, M.]] | [[Category: Vuckovic, M.]] | ||
[[Category: Zarivach, R.]] | [[Category: Zarivach, R.]] | ||
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[[Category: atpase]] | [[Category: atpase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:16:34 2008'' |
Revision as of 16:16, 21 February 2008
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Structural and biochemical analysis of a prototypical ATPase from the type III secretion system of pathogenic bacteria
Overview
The type III secretion system (T3SS) ATPase is the conserved and essential inner-membrane component involved in the initial stages of selective secretion of specialized T3SS virulence effector proteins from the bacterial cytoplasm through to the infected host cell, a process crucial to subsequent pathogenicity. Here we present the 1.8-A-resolution crystal structure of the catalytic domain of the prototypical T3SS ATPase EscN from enteropathogenic Escherichia coli (EPEC). Along with in vitro and in vivo mutational analysis, our data show that the T3SS ATPases share similarity with the F1 ATPases but have important structural and sequence differences that dictate their unique secretory role. We also show that T3SS ATPase activity is dependent on EscN oligomerization and describe the molecular features and possible functional implications of a hexameric ring model.
About this Structure
2OBL is a Single protein structure of sequence from Escherichia coli with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structural analysis of a prototypical ATPase from the type III secretion system., Zarivach R, Vuckovic M, Deng W, Finlay BB, Strynadka NC, Nat Struct Mol Biol. 2007 Feb;14(2):131-7. Epub 2007 Jan 21. PMID:17237797
Page seeded by OCA on Thu Feb 21 18:16:34 2008
Categories: Escherichia coli | Single protein | Deng, W. | Finlay, B B. | Strynadka, N C.J. | Vuckovic, M. | Zarivach, R. | ACT | CA | IMD | Atpase
