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2oiu
From Proteopedia
(New page: 200px<br /><applet load="2oiu" size="350" color="white" frame="true" align="right" spinBox="true" caption="2oiu, resolution 2.60Å" /> '''L1 Ribozyme Ligase c...) |
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==Overview== | ==Overview== | ||
| - | Life originated, according to the RNA World hypothesis, from | + | Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly. |
==About this Structure== | ==About this Structure== | ||
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The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17363667 17363667] | The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17363667 17363667] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Robertson, M | + | [[Category: Robertson, M P]] |
| - | [[Category: Scott, W | + | [[Category: Scott, W G.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: active site]] | [[Category: active site]] | ||
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[[Category: rna]] | [[Category: rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:01 2008'' |
Revision as of 16:19, 21 February 2008
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L1 Ribozyme Ligase circular adduct
Overview
Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.
About this Structure
2OIU is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:17363667
Page seeded by OCA on Thu Feb 21 18:19:01 2008
Categories: Protein complex | Robertson, M P | Scott, W G. | MG | Active site | In vitro selection | Ligase | Ribozyme | Rna
