2oiu

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(New page: 200px<br /><applet load="2oiu" size="350" color="white" frame="true" align="right" spinBox="true" caption="2oiu, resolution 2.60&Aring;" /> '''L1 Ribozyme Ligase c...)
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==Overview==
==Overview==
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Life originated, according to the RNA World hypothesis, from, self-replicating ribozymes that catalyzed ligation of RNA fragments. We, have solved the 2.6 angstrom crystal structure of a ligase ribozyme that, catalyzes regiospecific formation of a 5' to 3' phosphodiester bond, between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA, fragments. Invariant residues form tertiary contacts that stabilize a, flexible stem of the ribozyme at the ligation site, where an essential, magnesium ion coordinates three phosphates. The structure of the active, site permits us to suggest how transition-state stabilization and a, general base may catalyze the ligation reaction required for prebiotic RNA, assembly.
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Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.
==About this Structure==
==About this Structure==
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The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17363667 17363667]
The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17363667 17363667]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Robertson, M.P]]
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[[Category: Robertson, M P]]
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[[Category: Scott, W.G.]]
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[[Category: Scott, W G.]]
[[Category: MG]]
[[Category: MG]]
[[Category: active site]]
[[Category: active site]]
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[[Category: rna]]
[[Category: rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:11:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:01 2008''

Revision as of 16:19, 21 February 2008

Image:2oiu.gif

2oiu, resolution 2.60Å

Drag the structure with the mouse to rotate

L1 Ribozyme Ligase circular adduct

Overview

Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.

About this Structure

2OIU is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:17363667

Page seeded by OCA on Thu Feb 21 18:19:01 2008

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