2ojq
From Proteopedia
(New page: 200px<br /> <applet load="2ojq" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ojq, resolution 2.87Å" /> '''Crystal structure o...) |
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| - | [[Image:2ojq.gif|left|200px]]<br /> | + | [[Image:2ojq.gif|left|200px]]<br /><applet load="2ojq" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2ojq, resolution 2.87Å" /> | caption="2ojq, resolution 2.87Å" /> | ||
'''Crystal structure of Alix V domain'''<br /> | '''Crystal structure of Alix V domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The modular protein Alix is a central node in endosomal-lysosomal | + | The modular protein Alix is a central node in endosomal-lysosomal trafficking and the budding of human immunodeficiency virus (HIV)-1. The Gag p6 protein of HIV-1 contains a LYPx(n)LxxL motif that is required for Alix-mediated budding and binds a region of Alix spanning residues 360-702. The structure of this fragment of Alix has the shape of the letter 'V' and is termed the V domain. The V domain has a topologically complex arrangement of 11 alpha-helices, with connecting loops that cross three times between the two arms of the V. The conserved residue Phe676 is at the center of a large hydrophobic pocket and is crucial for binding to a peptide model of HIV-1 p6. Overexpression of the V domain inhibits HIV-1 release from cells. This inhibition of release is reversed by mutations that block binding of the Alix V domain to p6. |
==About this Structure== | ==About this Structure== | ||
| - | 2OJQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2OJQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OJQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hurley, J | + | [[Category: Hurley, J H.]] |
[[Category: Lee, S.]] | [[Category: Lee, S.]] | ||
[[Category: helical]] | [[Category: helical]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:14 2008'' |
Revision as of 16:19, 21 February 2008
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Crystal structure of Alix V domain
Overview
The modular protein Alix is a central node in endosomal-lysosomal trafficking and the budding of human immunodeficiency virus (HIV)-1. The Gag p6 protein of HIV-1 contains a LYPx(n)LxxL motif that is required for Alix-mediated budding and binds a region of Alix spanning residues 360-702. The structure of this fragment of Alix has the shape of the letter 'V' and is termed the V domain. The V domain has a topologically complex arrangement of 11 alpha-helices, with connecting loops that cross three times between the two arms of the V. The conserved residue Phe676 is at the center of a large hydrophobic pocket and is crucial for binding to a peptide model of HIV-1 p6. Overexpression of the V domain inhibits HIV-1 release from cells. This inhibition of release is reversed by mutations that block binding of the Alix V domain to p6.
About this Structure
2OJQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for viral late-domain binding to Alix., Lee S, Joshi A, Nagashima K, Freed EO, Hurley JH, Nat Struct Mol Biol. 2007 Mar;14(3):194-9. Epub 2007 Feb 4. PMID:17277784
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