2oki
From Proteopedia
(New page: 200px<br /><applet load="2oki" size="350" color="white" frame="true" align="right" spinBox="true" caption="2oki, resolution 2.70Å" /> '''Crystal structure of...) |
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==Overview== | ==Overview== | ||
| - | The crystals obtained from various batches of crystallization trials of | + | The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited non-isomorphism. The c axis of the I222 cell showed a large variation of about 16 A, from c = 81 A to c = 97 A. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, c = 97 A; form 2, c = 92 A; form 3, c = 81 A). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Padala, P.]] | [[Category: Padala, P.]] | ||
| - | [[Category: Sharma, S | + | [[Category: Sharma, S K.]] |
[[Category: Suguna, K.]] | [[Category: Suguna, K.]] | ||
[[Category: Surolia, A.]] | [[Category: Surolia, A.]] | ||
[[Category: Surolia, N.]] | [[Category: Surolia, N.]] | ||
| - | [[Category: Swarnamukhi, P | + | [[Category: Swarnamukhi, P L.]] |
[[Category: fabz]] | [[Category: fabz]] | ||
[[Category: hotdog fold]] | [[Category: hotdog fold]] | ||
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[[Category: plasmodium]] | [[Category: plasmodium]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:33 2008'' |
Revision as of 16:19, 21 February 2008
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Crystal structure of dimeric form of PfFabZ in crystal form2
Overview
The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited non-isomorphism. The c axis of the I222 cell showed a large variation of about 16 A, from c = 81 A to c = 97 A. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, c = 97 A; form 2, c = 92 A; form 3, c = 81 A). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences.
About this Structure
2OKI is a Single protein structure of sequence from Plasmodium falciparum. Active as [acyl-carrier-protein_dehydratase 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase], with EC number 4.2.1.60 Full crystallographic information is available from OCA.
Reference
Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum., Swarnamukhi PL, Sharma SK, Padala P, Surolia N, Surolia A, Suguna K, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):458-64. Epub 2007, Mar 16. PMID:17372349 [[Category: 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase]]
Page seeded by OCA on Thu Feb 21 18:19:33 2008
