2okr
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The p38 signaling pathway is activated in response to cell stress and | + | The p38 signaling pathway is activated in response to cell stress and induces production of proinflammatory cytokines. P38alpha is phosphorylated and activated in response to cell stress by MKK3 and MKK6 and in turn phosphorylates a number of substrates, including MAPKAP kinase 2 (MK2). We have determined the crystal structure of the unphosphorylated p38alpha-MK2 heterodimer. The C-terminal regulatory domain of MK2 binds in the docking groove of p38alpha, and the ATP-binding sites of both kinases are at the heterodimer interface. The conformation suggests an extra mechanism in addition to the regulation of the p38alpha and MK2 phosphorylation states that prevents phosphorylation of substrates in the absence of cell stress. Addition of constitutively active MKK6-DD results in rapid phosphorylation of the p38alpha-MK2 heterodimer. |
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
| - | Crystal structure of the | + | Crystal structure of the p38 alpha-MAPKAP kinase 2 heterodimer., ter Haar E, Prabhakar P, Liu X, Lepre C, J Biol Chem. 2007 Mar 30;282(13):9733-9. Epub 2007 Jan 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17255097 17255097] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Mitogen-activated protein kinase]] | [[Category: Mitogen-activated protein kinase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Haar, E | + | [[Category: Haar, E Ter.]] |
[[Category: docking groove]] | [[Category: docking groove]] | ||
[[Category: heterodimer]] | [[Category: heterodimer]] | ||
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[[Category: nls]] | [[Category: nls]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:33 2008'' |
Revision as of 16:19, 21 February 2008
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Crystal Structure of the P38a-MAPKAP kinase 2 Heterodimer
Overview
The p38 signaling pathway is activated in response to cell stress and induces production of proinflammatory cytokines. P38alpha is phosphorylated and activated in response to cell stress by MKK3 and MKK6 and in turn phosphorylates a number of substrates, including MAPKAP kinase 2 (MK2). We have determined the crystal structure of the unphosphorylated p38alpha-MK2 heterodimer. The C-terminal regulatory domain of MK2 binds in the docking groove of p38alpha, and the ATP-binding sites of both kinases are at the heterodimer interface. The conformation suggests an extra mechanism in addition to the regulation of the p38alpha and MK2 phosphorylation states that prevents phosphorylation of substrates in the absence of cell stress. Addition of constitutively active MKK6-DD results in rapid phosphorylation of the p38alpha-MK2 heterodimer.
About this Structure
2OKR is a Protein complex structure of sequences from Homo sapiens. Active as Mitogen-activated protein kinase, with EC number 2.7.11.24 Full crystallographic information is available from OCA.
Reference
Crystal structure of the p38 alpha-MAPKAP kinase 2 heterodimer., ter Haar E, Prabhakar P, Liu X, Lepre C, J Biol Chem. 2007 Mar 30;282(13):9733-9. Epub 2007 Jan 25. PMID:17255097
Page seeded by OCA on Thu Feb 21 18:19:33 2008
