2opc
From Proteopedia
(New page: 200px<br /><applet load="2opc" size="350" color="white" frame="true" align="right" spinBox="true" caption="2opc, resolution 1.43Å" /> '''Structure of Melamps...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | Metal-binding sites are ubiquitous in proteins and can be readily utilized | + | Metal-binding sites are ubiquitous in proteins and can be readily utilized for phasing. It is shown that a protein crystal structure can be solved using single-wavelength anomalous diffraction based on the anomalous signal of a cobalt ion measured on a conventional monochromatic X-ray source. The unique absorption edge of cobalt (1.61 A) is compatible with the Cu K alpha wavelength (1.54 A) commonly available in macromolecular crystallography laboratories. This approach was applied to the determination of the structure of Melampsora lini avirulence protein AvrL567-A, a protein with a novel fold from the fungal pathogen flax rust that induces plant disease resistance in flax plants. This approach using cobalt ions may be applicable to all cobalt-binding proteins and may be advantageous when synchrotron radiation is not readily available. |
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
| - | The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein., Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B, Acta | + | The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein., Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):209-13. Epub 2007 Feb 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17329816 17329816] |
[[Category: Melampsora lini]] | [[Category: Melampsora lini]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Catanzariti, A | + | [[Category: Catanzariti, A M.]] |
| - | [[Category: Dodds, P | + | [[Category: Dodds, P N.]] |
| - | [[Category: Ellis, J | + | [[Category: Ellis, J G.]] |
| - | [[Category: Forwood, J | + | [[Category: Forwood, J K.]] |
[[Category: Guncar, G.]] | [[Category: Guncar, G.]] | ||
[[Category: Kobe, B.]] | [[Category: Kobe, B.]] | ||
[[Category: Teh, T.]] | [[Category: Teh, T.]] | ||
| - | [[Category: Wang, C | + | [[Category: Wang, C I.]] |
[[Category: CO]] | [[Category: CO]] | ||
[[Category: IMD]] | [[Category: IMD]] | ||
| Line 29: | Line 29: | ||
[[Category: single-wavelength anomalous dispersion (sad)]] | [[Category: single-wavelength anomalous dispersion (sad)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:21:02 2008'' |
Revision as of 16:21, 21 February 2008
|
Structure of Melampsora lini avirulence protein, AvrL567-A
Overview
Metal-binding sites are ubiquitous in proteins and can be readily utilized for phasing. It is shown that a protein crystal structure can be solved using single-wavelength anomalous diffraction based on the anomalous signal of a cobalt ion measured on a conventional monochromatic X-ray source. The unique absorption edge of cobalt (1.61 A) is compatible with the Cu K alpha wavelength (1.54 A) commonly available in macromolecular crystallography laboratories. This approach was applied to the determination of the structure of Melampsora lini avirulence protein AvrL567-A, a protein with a novel fold from the fungal pathogen flax rust that induces plant disease resistance in flax plants. This approach using cobalt ions may be applicable to all cobalt-binding proteins and may be advantageous when synchrotron radiation is not readily available.
About this Structure
2OPC is a Single protein structure of sequence from Melampsora lini with and as ligands. Full crystallographic information is available from OCA.
Reference
The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein., Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):209-13. Epub 2007 Feb 23. PMID:17329816
Page seeded by OCA on Thu Feb 21 18:21:02 2008
