4bl0

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{{STRUCTURE_4bl0| PDB=4bl0 | SCENE= }}
{{STRUCTURE_4bl0| PDB=4bl0 | SCENE= }}
===Crystal structure of yeast Bub3-Bub1 bound to phospho-Spc105===
===Crystal structure of yeast Bub3-Bub1 bound to phospho-Spc105===
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{{ABSTRACT_PUBMED_24066227}}
==Function==
==Function==
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==Reference==
==Reference==
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<references group="xtra"/><references/>
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<ref group="xtra">PMID:024066227</ref><references group="xtra"/><references/>
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]

Revision as of 05:11, 10 October 2013

Template:STRUCTURE 4bl0

Contents

Crystal structure of yeast Bub3-Bub1 bound to phospho-Spc105

Template:ABSTRACT PUBMED 24066227

Function

[BUB3_YEAST] Required for cell cycle arrest in response to loss of microtubule function. Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20. The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism.[1] [SP105_YEAST] Forms a kinetochore complex with SPC105 which is required for kinetochore binding by a discrete subset of kMAPs (BIM1, BIK1 and SLK19) and motors (CIN8, KAR3). Involved in kinetochore-microtubule binding and the spindle assembly checkpoint.[2] [BUB1_YEAST] Involved in cell cycle checkpoint enforcement. The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism. Catalyzes the phosphorylation of BUB3 and its autophosphorylation. Associates with centromere (CEN) DNA via interaction with SKP1. The association with SKP1 is required for the mitotic delay induced by kinetochore tension defects, but not for the arrest induced by spindle depolymerization or kinetochore assembly defects.[3]

About this Structure

4bl0 is a 6 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

  • Primorac I, Weir JR, Chiroli E, Gross F, Hoffmann I, van Gerwen S, Ciliberto A, Musacchio A. Bub3 reads phosphorylated MELT repeats to promote spindle assembly checkpoint signaling. Elife. 2013 Sep 24;2:e01030. doi: 10.7554/eLife.01030. PMID:24066227 doi:http://dx.doi.org/10.7554/eLife.01030
  1. ↑ Poddar A, Stukenberg PT, Burke DJ. Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae. Eukaryot Cell. 2005 May;4(5):867-78. PMID:15879521 doi:10.1128/EC.4.5.867-878.2005
  2. ↑ Pagliuca C, Draviam VM, Marco E, Sorger PK, De Wulf P. Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint. PLoS One. 2009 Oct 28;4(10):e7640. PMID:19893618 doi:10.1371/journal.pone.0007640
  3. ↑ Kitagawa K, Abdulle R, Bansal PK, Cagney G, Fields S, Hieter P. Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint. Mol Cell. 2003 May;11(5):1201-13. PMID:12769845

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