2ost
From Proteopedia
(New page: 200px<br /><applet load="2ost" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ost, resolution 3.100Å" /> '''The structure of a ...) |
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==Overview== | ==Overview== | ||
| - | The homing endonuclease I-Ssp6803I causes the insertion of a group I | + | The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Synechocystis sp.]] | [[Category: Synechocystis sp.]] | ||
| - | [[Category: Bonocora, R | + | [[Category: Bonocora, R P.]] |
| - | [[Category: Shub, D | + | [[Category: Shub, D A.]] |
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: Zhao, L.]] | [[Category: Zhao, L.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: restriction enzyme fold]] | [[Category: restriction enzyme fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:22:06 2008'' |
Revision as of 16:22, 21 February 2008
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The structure of a bacterial homing endonuclease : I-Ssp6803I
Overview
The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.
About this Structure
2OST is a Single protein structure of sequence from Synechocystis sp. with as ligand. Full crystallographic information is available from OCA.
Reference
The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif., Zhao L, Bonocora RP, Shub DA, Stoddard BL, EMBO J. 2007 May 2;26(9):2432-42. Epub 2007 Apr 5. PMID:17410205
Page seeded by OCA on Thu Feb 21 18:22:06 2008
