2otj
From Proteopedia
(New page: 200px<br /><applet load="2otj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2otj, resolution 2.900Å" /> '''13-deoxytedanolide ...) |
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| - | [[Image:2otj.jpg|left|200px]]<br /><applet load="2otj" size=" | + | [[Image:2otj.jpg|left|200px]]<br /><applet load="2otj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="2otj, resolution 2.900Å" /> | caption="2otj, resolution 2.900Å" /> | ||
'''13-deoxytedanolide bound to the large subunit of Haloarcula marismortui'''<br /> | '''13-deoxytedanolide bound to the large subunit of Haloarcula marismortui'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Crystal structures of the 50 S ribosomal subunit from Haloarcula | + | Crystal structures of the 50 S ribosomal subunit from Haloarcula marismortui complexed with two antibiotics have identified new sites at which antibiotics interact with the ribosome and inhibit protein synthesis. 13-Deoxytedanolide binds to the E site of the 50 S subunit at the same location as the CCA of tRNA, and thus appears to inhibit protein synthesis by competing with deacylated tRNAs for E site binding. Girodazole binds near the E site region, but is somewhat buried and may inhibit tRNA binding by interfering with conformational changes that occur at the E site. The specificity of 13-deoxytedanolide for eukaryotic ribosomes is explained by its extensive interactions with protein L44e, which is an E site component of archaeal and eukaryotic ribosomes, but not of eubacterial ribosomes. In addition, protein L28, which is unique to the eubacterial E site, overlaps the site occupied by 13-deoxytedanolide, precluding its binding to eubacterial ribosomes. Girodazole is specific for eukarytes and archaea because it makes interactions with L15 that are not possible in eubacteria. |
==About this Structure== | ==About this Structure== | ||
| - | 2OTJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with 13T, MG, K, NA, CD and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2OTJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with <scene name='pdbligand=13T:'>13T</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTJ OCA]. |
==Reference== | ==Reference== | ||
| - | The | + | The structures of antibiotics bound to the E site region of the 50 S ribosomal subunit of Haloarcula marismortui: 13-deoxytedanolide and girodazole., Schroeder SJ, Blaha G, Tirado-Rives J, Steitz TA, Moore PB, J Mol Biol. 2007 Apr 13;367(5):1471-9. Epub 2007 Feb 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17321546 17321546] |
[[Category: Haloarcula marismortui]] | [[Category: Haloarcula marismortui]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Blaha, G.]] | [[Category: Blaha, G.]] | ||
| - | [[Category: Schroeder, S | + | [[Category: Schroeder, S J.]] |
[[Category: Tirado-Rives, J.]] | [[Category: Tirado-Rives, J.]] | ||
[[Category: 13T]] | [[Category: 13T]] | ||
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[[Category: ribosome]] | [[Category: ribosome]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:22:20 2008'' |
Revision as of 16:22, 21 February 2008
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13-deoxytedanolide bound to the large subunit of Haloarcula marismortui
Overview
Crystal structures of the 50 S ribosomal subunit from Haloarcula marismortui complexed with two antibiotics have identified new sites at which antibiotics interact with the ribosome and inhibit protein synthesis. 13-Deoxytedanolide binds to the E site of the 50 S subunit at the same location as the CCA of tRNA, and thus appears to inhibit protein synthesis by competing with deacylated tRNAs for E site binding. Girodazole binds near the E site region, but is somewhat buried and may inhibit tRNA binding by interfering with conformational changes that occur at the E site. The specificity of 13-deoxytedanolide for eukaryotic ribosomes is explained by its extensive interactions with protein L44e, which is an E site component of archaeal and eukaryotic ribosomes, but not of eubacterial ribosomes. In addition, protein L28, which is unique to the eubacterial E site, overlaps the site occupied by 13-deoxytedanolide, precluding its binding to eubacterial ribosomes. Girodazole is specific for eukarytes and archaea because it makes interactions with L15 that are not possible in eubacteria.
About this Structure
2OTJ is a Protein complex structure of sequences from Haloarcula marismortui with , , , , and as ligands. Full crystallographic information is available from OCA.
Reference
The structures of antibiotics bound to the E site region of the 50 S ribosomal subunit of Haloarcula marismortui: 13-deoxytedanolide and girodazole., Schroeder SJ, Blaha G, Tirado-Rives J, Steitz TA, Moore PB, J Mol Biol. 2007 Apr 13;367(5):1471-9. Epub 2007 Feb 7. PMID:17321546
Page seeded by OCA on Thu Feb 21 18:22:20 2008
Categories: Haloarcula marismortui | Protein complex | Blaha, G. | Schroeder, S J. | Tirado-Rives, J. | 13T | CD | CL | K | MG | NA | 13 deoxytedanolide | 50s | Antibiotic complex | Ribosome
