2ovn
From Proteopedia
(New page: 200px<br /><applet load="2ovn" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ovn" /> '''NMR structure of the GCN4 trigger peptide'''...) |
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==Overview== | ==Overview== | ||
| - | Coiled coils have attracted considerable interest as design templates in a | + | Coiled coils have attracted considerable interest as design templates in a wide range of applications. Successful coiled-coil design strategies therefore require a detailed understanding of coiled-coil folding. One common feature shared by coiled coils is the presence of a short autonomous helical folding unit, termed "trigger sequence," that is indispensable for folding. Detailed knowledge of trigger sequences at the molecular level is thus key to a general understanding of coiled-coil formation. Using a multidisciplinary approach, we identify and characterize here the molecular determinants that specify the helical conformation of the monomeric early folding intermediate of the GCN4 coiled coil. We demonstrate that a network of hydrogen-bonding and electrostatic interactions stabilize the trigger-sequence helix. This network is rearranged in the final dimeric coiled-coil structure, and its destabilization significantly slows down GCN4 leucine zipper folding. Our findings provide a general explanation for the molecular mechanism of coiled-coil formation. |
==About this Structure== | ==About this Structure== | ||
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Molecular basis of coiled-coil formation., Steinmetz MO, Jelesarov I, Matousek WM, Honnappa S, Jahnke W, Missimer JH, Frank S, Alexandrescu AT, Kammerer RA, Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7062-7. Epub 2007 Apr 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17438295 17438295] | Molecular basis of coiled-coil formation., Steinmetz MO, Jelesarov I, Matousek WM, Honnappa S, Jahnke W, Missimer JH, Frank S, Alexandrescu AT, Kammerer RA, Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7062-7. Epub 2007 Apr 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17438295 17438295] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Alexandrescu, A | + | [[Category: Alexandrescu, A T.]] |
| - | [[Category: Matousek, W | + | [[Category: Matousek, W M.]] |
[[Category: coiled-coil]] | [[Category: coiled-coil]] | ||
[[Category: gcn4]] | [[Category: gcn4]] | ||
[[Category: trigger peptide]] | [[Category: trigger peptide]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:23:05 2008'' |
Revision as of 16:23, 21 February 2008
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NMR structure of the GCN4 trigger peptide
Overview
Coiled coils have attracted considerable interest as design templates in a wide range of applications. Successful coiled-coil design strategies therefore require a detailed understanding of coiled-coil folding. One common feature shared by coiled coils is the presence of a short autonomous helical folding unit, termed "trigger sequence," that is indispensable for folding. Detailed knowledge of trigger sequences at the molecular level is thus key to a general understanding of coiled-coil formation. Using a multidisciplinary approach, we identify and characterize here the molecular determinants that specify the helical conformation of the monomeric early folding intermediate of the GCN4 coiled coil. We demonstrate that a network of hydrogen-bonding and electrostatic interactions stabilize the trigger-sequence helix. This network is rearranged in the final dimeric coiled-coil structure, and its destabilization significantly slows down GCN4 leucine zipper folding. Our findings provide a general explanation for the molecular mechanism of coiled-coil formation.
About this Structure
2OVN is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Molecular basis of coiled-coil formation., Steinmetz MO, Jelesarov I, Matousek WM, Honnappa S, Jahnke W, Missimer JH, Frank S, Alexandrescu AT, Kammerer RA, Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7062-7. Epub 2007 Apr 16. PMID:17438295
Page seeded by OCA on Thu Feb 21 18:23:05 2008
