2ozy
From Proteopedia
(New page: 200px<br /><applet load="2ozy" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ozy, resolution 1.740Å" /> '''Crystal structure o...) |
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==Overview== | ==Overview== | ||
| - | NrfB is a small pentahaem electron-transfer protein widely involved in the | + | NrfB is a small pentahaem electron-transfer protein widely involved in the respiratory reduction of nitrite or nitric oxide to ammonia, processes that provide energy for anaerobic metabolism in many enteric bacteria and also serve to detoxify these reactive nitrogen species. The X-ray crystal structure of Escherichia coli NrfB is presented at 1.74 A (1 A=0.1 nm) resolution. The architecture of the protein is that of a 40 A 'nanowire' in which the five haems are positioned within 6 A of each other along a polypeptide scaffold. During nitrite reduction, the physiological role of NrfB is to mediate electron transfer to another pentahaem protein, NrfA, the enzyme that catalyses periplasmic nitrite or nitric oxide reduction. Protein-protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2-NrfB2 heterotetrameric complex. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Clarke, T | + | [[Category: Clarke, T A.]] |
| - | [[Category: Hemmings, A | + | [[Category: Hemmings, A M.]] |
| - | [[Category: Richardson, D | + | [[Category: Richardson, D J.]] |
[[Category: HEC]] | [[Category: HEC]] | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
[[Category: pentaheme c-type cytochrome]] | [[Category: pentaheme c-type cytochrome]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:24:22 2008'' |
Revision as of 16:24, 21 February 2008
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Crystal structure of E.coli nrfB
Overview
NrfB is a small pentahaem electron-transfer protein widely involved in the respiratory reduction of nitrite or nitric oxide to ammonia, processes that provide energy for anaerobic metabolism in many enteric bacteria and also serve to detoxify these reactive nitrogen species. The X-ray crystal structure of Escherichia coli NrfB is presented at 1.74 A (1 A=0.1 nm) resolution. The architecture of the protein is that of a 40 A 'nanowire' in which the five haems are positioned within 6 A of each other along a polypeptide scaffold. During nitrite reduction, the physiological role of NrfB is to mediate electron transfer to another pentahaem protein, NrfA, the enzyme that catalyses periplasmic nitrite or nitric oxide reduction. Protein-protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2-NrfB2 heterotetrameric complex.
About this Structure
2OZY is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA., Clarke TA, Cole JA, Richardson DJ, Hemmings AM, Biochem J. 2007 Aug 15;406(1):19-30. PMID:17521287
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