2p0c

From Proteopedia

Revision as of 16:24, 21 February 2008

Catalytic Domain of the Proto-oncogene Tyrosine-protein Kinase MER

Overview

A human B-lymphoblastoid lambda gt11 expression library was screened using anti-phosphotyrosine antibodies yielding complementary DNAs encoding active tyrosine kinases. The resulting clones were used to obtain the sequence of a novel 984 amino acid transmembrane tyrosine kinase. Analysis of the complementary DNA revealed extracellular immunoglobulin and fibronectin type III domains and the unusual kinase signature sequence KWIAIES; all are characteristic of the axl family of tyrosine kinases. The novel tyrosine kinase was not expressed in normal B- and T-lymphocytes but, unlike axl, was expressed in numerous neoplastic B- and T-cell lines. Transcripts for the novel receptor-like tyrosine kinase were detected in normal peripheral blood monocytes and bone marrow. One alternatively spliced transcript was detected which contained an insert in the membrane proximal region that could encode for a truncated, soluble receptor. Sequence comparison shows that the kinase may be the human protooncogene for the recently isolated chicken retroviral oncogene v-ryk (recently renamed v-eyk), a truncated tyrosine kinase whose expression by retroviral infection produced sarcomas in chickens. The intracellular domain of the human kinase shows 83% similarity and 71% identity to v-ryk. Since the ryk designation has been used to name another tyrosine kinase and an analysis of RNA expression demonstrated that this novel human kinase is expressed in monocytes and tissues of epithelial and reproductive origin, we have designated our novel protooncogene c-mer.

Disease

Known diseases associated with this structure: Blood group, Raph OMIM:[602243], Nephropathy with pretibial epidermolysis bullosa and deafness OMIM:[602243], Retinitis pigmentosa-38 OMIM:[604705]

About this Structure

2P0C is a Single protein structure of sequence from Homo sapiens with , and as ligands. Active as Receptor protein-tyrosine kinase, with EC number 2.7.10.1 Full crystallographic information is available from OCA.

Reference

Cloning and mRNA expression analysis of a novel human protooncogene, c-mer., Graham DK, Dawson TL, Mullaney DL, Snodgrass HR, Earp HS, Cell Growth Differ. 1994 Jun;5(6):647-57. PMID:8086340

Page seeded by OCA on Thu Feb 21 18:24:34 2008