2p4w
From Proteopedia
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==Overview== | ==Overview== | ||
| - | We report here the crystal structure of a protein from Pyrococcus furiosus | + | We report here the crystal structure of a protein from Pyrococcus furiosus (Phr) that represents the first characterized heat shock transcription factor in archaea. Phr specifically represses the expression of heat shock genes at physiological temperature in vitro and in vivo but is released from the promoters upon heat shock response. Structure analysis revealed a stable homodimer, each subunit consisting of an N-terminal winged helix DNA-binding domain (wH-DBD) and a C-terminal antiparallel coiled coil helical domain. The overall structure shows as a molecular chimera with significant folding similarity of its DBD to the bacterial SmtB/ArsR family, while its C-terminal part was found to be a remote homologue of the eukaryotic BAG domain. The dimeric protein recognizes a palindromic DNA sequence. Molecular docking and mutational analyses suggested a novel binding mode in which the major specific contacts occur at the minor groove interacting with the strongly basic wing containing a cluster of three arginine residues. |
==About this Structure== | ==About this Structure== | ||
| - | 2P4W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure | + | 2P4W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 1XNP. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P4W OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal | + | Crystal structure of the archaeal heat shock regulator from Pyrococcus furiosus: a molecular chimera representing eukaryal and bacterial features., Liu W, Vierke G, Wenke AK, Thomm M, Ladenstein R, J Mol Biol. 2007 Jun 1;369(2):474-88. Epub 2007 Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17434531 17434531] |
[[Category: Pyrococcus furiosus]] | [[Category: Pyrococcus furiosus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: winged helix]] | [[Category: winged helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:25:52 2008'' |
Revision as of 16:25, 21 February 2008
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Crystal structure of heat shock regulator from Pyrococcus furiosus
Overview
We report here the crystal structure of a protein from Pyrococcus furiosus (Phr) that represents the first characterized heat shock transcription factor in archaea. Phr specifically represses the expression of heat shock genes at physiological temperature in vitro and in vivo but is released from the promoters upon heat shock response. Structure analysis revealed a stable homodimer, each subunit consisting of an N-terminal winged helix DNA-binding domain (wH-DBD) and a C-terminal antiparallel coiled coil helical domain. The overall structure shows as a molecular chimera with significant folding similarity of its DBD to the bacterial SmtB/ArsR family, while its C-terminal part was found to be a remote homologue of the eukaryotic BAG domain. The dimeric protein recognizes a palindromic DNA sequence. Molecular docking and mutational analyses suggested a novel binding mode in which the major specific contacts occur at the minor groove interacting with the strongly basic wing containing a cluster of three arginine residues.
About this Structure
2P4W is a Single protein structure of sequence from Pyrococcus furiosus with as ligand. This structure supersedes the now removed PDB entry 1XNP. Full crystallographic information is available from OCA.
Reference
Crystal structure of the archaeal heat shock regulator from Pyrococcus furiosus: a molecular chimera representing eukaryal and bacterial features., Liu W, Vierke G, Wenke AK, Thomm M, Ladenstein R, J Mol Biol. 2007 Jun 1;369(2):474-88. Epub 2007 Mar 24. PMID:17434531
Page seeded by OCA on Thu Feb 21 18:25:52 2008
