2pc6
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Crystal structures of two orthologs of the regulatory subunit of | + | Crystal structures of two orthologs of the regulatory subunit of acetohydroxyacid synthase III (AHAS, EC 2.2.1.6) from Thermotoga maritima (TM0549) and Nitrosomonas europea (NE1324) were determined by single-wavelength anomalous diffraction methods with the use of selenomethionine derivatives at 2.3 A and 2.5 A, respectively. TM0549 and NE1324 share the same fold, and in both proteins the polypeptide chain contains two separate domains of a similar size. Each protein contains a C-terminal domain with ferredoxin-type fold and an N-terminal ACT domain, of which the latter is characteristic for several proteins involved in amino acid metabolism. The ferredoxin domain is stabilized by a calcium ion in the crystal structure of NE1324 and by a Mg(H2O)(6)2+ ion in TM0549. Both TM0549 and NE1324 form dimeric assemblies in the crystal lattice. |
==About this Structure== | ==About this Structure== | ||
| - | 2PC6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea_atcc_19718 Nitrosomonas europaea atcc 19718] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=SIN:'>SIN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | + | 2PC6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea_atcc_19718 Nitrosomonas europaea atcc 19718] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=SIN:'>SIN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 2FGD. Active as [http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PC6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chruszcz, M.]] | [[Category: Chruszcz, M.]] | ||
| - | [[Category: Cymborowski, M | + | [[Category: Cymborowski, M T.]] |
[[Category: Edwards, A.]] | [[Category: Edwards, A.]] | ||
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
[[Category: Minor, W.]] | [[Category: Minor, W.]] | ||
[[Category: Onopriyenko, O.]] | [[Category: Onopriyenko, O.]] | ||
| - | [[Category: Petkowski, J | + | [[Category: Petkowski, J J.]] |
[[Category: Savchenko, A.]] | [[Category: Savchenko, A.]] | ||
[[Category: Skarina, T.]] | [[Category: Skarina, T.]] | ||
[[Category: Zheng, H.]] | [[Category: Zheng, H.]] | ||
| - | [[Category: Zimmerman, M | + | [[Category: Zimmerman, M D.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: SIN]] | [[Category: SIN]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:28:05 2008'' |
Revision as of 16:28, 21 February 2008
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Crystal structure of putative acetolactate synthase- small subunit from Nitrosomonas europaea
Overview
Crystal structures of two orthologs of the regulatory subunit of acetohydroxyacid synthase III (AHAS, EC 2.2.1.6) from Thermotoga maritima (TM0549) and Nitrosomonas europea (NE1324) were determined by single-wavelength anomalous diffraction methods with the use of selenomethionine derivatives at 2.3 A and 2.5 A, respectively. TM0549 and NE1324 share the same fold, and in both proteins the polypeptide chain contains two separate domains of a similar size. Each protein contains a C-terminal domain with ferredoxin-type fold and an N-terminal ACT domain, of which the latter is characteristic for several proteins involved in amino acid metabolism. The ferredoxin domain is stabilized by a calcium ion in the crystal structure of NE1324 and by a Mg(H2O)(6)2+ ion in TM0549. Both TM0549 and NE1324 form dimeric assemblies in the crystal lattice.
About this Structure
2PC6 is a Single protein structure of sequence from Nitrosomonas europaea atcc 19718 with and as ligands. This structure supersedes the now removed PDB entry 2FGD. Active as Acetolactate synthase, with EC number 2.2.1.6 Full crystallographic information is available from OCA.
Reference
Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit., Petkowski JJ, Chruszcz M, Zimmerman MD, Zheng H, Skarina T, Onopriyenko O, Cymborowski MT, Koclega KD, Savchenko A, Edwards A, Minor W, Protein Sci. 2007 Jul;16(7):1360-7. PMID:17586771
Page seeded by OCA on Thu Feb 21 18:28:05 2008
Categories: Acetolactate synthase | Nitrosomonas europaea atcc 19718 | Single protein | Chruszcz, M. | Cymborowski, M T. | Edwards, A. | Joachimiak, A. | MCSG, Midwest Center for Structural Genomics. | Minor, W. | Onopriyenko, O. | Petkowski, J J. | Savchenko, A. | Skarina, T. | Zheng, H. | Zimmerman, M D. | CA | SIN | Lyase | Mcsg | Midwest center for structural genomics | Protein structure initiative | Psi | Regulatory subunit | Structural genomics
