4mdx

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'''Unreleased structure'''
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{{STRUCTURE_4mdx| PDB=4mdx | SCENE= }}
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===Crystal structure of Bacillus subtilis MazF in complex with RNA===
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The entry 4mdx is ON HOLD until Paper Publication
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==Function==
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[[http://www.uniprot.org/uniprot/ENDOA_BACSU ENDOA_BACSU]] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.<ref>PMID:15882409</ref> <ref>PMID:21763692</ref>
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Authors: Simanshu, D.K., Patel, D.J.
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==About this Structure==
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[[4mdx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDX OCA].
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Description: Crystal structure of Bacillus subtilis MazF in complex with RNA
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==Reference==
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<references group="xtra"/><references/>
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[[Category: Bacillus subtilis subsp. subtilis str. 168]]
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[[Category: Patel, D J.]]
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[[Category: Simanshu, D K.]]
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[[Category: Antitoxin]]
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[[Category: Endoa]]
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[[Category: Hydrolase-rna complex]]
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[[Category: Maze]]
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[[Category: Mazf]]
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[[Category: Mrna interferase]]
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[[Category: Toxin]]
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[[Category: Toxin-antitoxin complex]]
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[[Category: Toxin-mrna complex]]
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[[Category: Ydce]]

Revision as of 06:56, 23 October 2013

Template:STRUCTURE 4mdx

Contents

Crystal structure of Bacillus subtilis MazF in complex with RNA

Function

[ENDOA_BACSU] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.[1] [2]

About this Structure

4mdx is a 3 chain structure with sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA.

Reference

  1. Pellegrini O, Mathy N, Gogos A, Shapiro L, Condon C. The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Mol Microbiol. 2005 Jun;56(5):1139-48. PMID:15882409 doi:MMI4606
  2. Park JH, Yamaguchi Y, Inouye M. Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase. FEBS Lett. 2011 Aug 4;585(15):2526-32. doi: 10.1016/j.febslet.2011.07.008. Epub, 2011 Jul 13. PMID:21763692 doi:http://dx.doi.org/10.1016/j.febslet.2011.07.008

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