2pfg
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Glutathione forms complex reaction products with formaldehyde, which can | + | Glutathione forms complex reaction products with formaldehyde, which can be further modified through enzymatic modification. We studied the non-enzymatic reaction between glutathione and formaldehyde and identified a bicyclic complex containing two equivalents of formaldehyde and one glutathione molecule by protein X-ray crystallography (PDB accession number 2PFG). We have also used (1)H, (13)C and 2D NMR spectroscopy to confirm the structure of this unusual adduct. The key feature of this adduct is the involvement of the gamma-glutamyl alpha-amine and the Cys thiol in the formation of the bicyclic ring structure. These findings suggest that the structure of GSH allows for bi-dentate masking of the reactivity of formaldehyde. As this species predominates at near physiological pH values, we suggest this adduct may have biological significance. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bateman, R | + | [[Category: Bateman, R L.]] |
[[Category: Rauh, D.]] | [[Category: Rauh, D.]] | ||
| - | [[Category: Shokat, K | + | [[Category: Shokat, K M.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: DDD]] | [[Category: DDD]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:28:51 2008'' |
Revision as of 16:28, 21 February 2008
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Crystal structure of human CBR1 in complex with BiGF2.
Overview
Glutathione forms complex reaction products with formaldehyde, which can be further modified through enzymatic modification. We studied the non-enzymatic reaction between glutathione and formaldehyde and identified a bicyclic complex containing two equivalents of formaldehyde and one glutathione molecule by protein X-ray crystallography (PDB accession number 2PFG). We have also used (1)H, (13)C and 2D NMR spectroscopy to confirm the structure of this unusual adduct. The key feature of this adduct is the involvement of the gamma-glutamyl alpha-amine and the Cys thiol in the formation of the bicyclic ring structure. These findings suggest that the structure of GSH allows for bi-dentate masking of the reactivity of formaldehyde. As this species predominates at near physiological pH values, we suggest this adduct may have biological significance.
About this Structure
2PFG is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Glutathione traps formaldehyde by formation of a bicyclo[4.4.1]undecane adduct., Bateman R, Rauh D, Shokat KM, Org Biomol Chem. 2007 Oct 21;5(20):3363-7. Epub 2007 Aug 29. PMID:17912391
Page seeded by OCA on Thu Feb 21 18:28:51 2008
Categories: Homo sapiens | Single protein | Bateman, R L. | Rauh, D. | Shokat, K M. | CL | DDD | GOL | NAP | Glutathione | Macro molecule | Oxidoreductase
