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2phw
From Proteopedia
(New page: 200px<br /><applet load="2phw" size="350" color="white" frame="true" align="right" spinBox="true" caption="2phw, resolution 1.8Å" /> '''Pterocarpus angolensi...) |
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==Overview== | ==Overview== | ||
| - | The crystal structure of Pterocarpus angolensis seed lectin is presented | + | The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context. |
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
| - | How a | + | How a plant lectin recognizes high mannose oligosaccharides., Garcia-Pino A, Buts L, Wyns L, Imberty A, Loris R, Plant Physiol. 2007 Aug;144(4):1733-41. Epub 2007 Jun 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17556509 17556509] |
[[Category: Pterocarpus angolensis]] | [[Category: Pterocarpus angolensis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: lectin]] | [[Category: lectin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:29:37 2008'' |
Revision as of 16:29, 21 February 2008
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Pterocarpus angolensis lectin (PAL) in complex with Man-9
Overview
The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.
About this Structure
2PHW is a Single protein structure of sequence from Pterocarpus angolensis with and as ligands. Full crystallographic information is available from OCA.
Reference
How a plant lectin recognizes high mannose oligosaccharides., Garcia-Pino A, Buts L, Wyns L, Imberty A, Loris R, Plant Physiol. 2007 Aug;144(4):1733-41. Epub 2007 Jun 7. PMID:17556509
Page seeded by OCA on Thu Feb 21 18:29:37 2008
Categories: Pterocarpus angolensis | Single protein | Buts, L. | Garcia-Pino, A. | Imberty, A. | Loris, R. | Wyns, L. | CA | MN | Carbohydrate | High mannose | Lectin
