2plb

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(New page: 200px<br /><applet load="2plb" size="350" color="white" frame="true" align="right" spinBox="true" caption="2plb, resolution 1.60&Aring;" /> '''D(GTATACC) under hyd...)
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==Overview==
==Overview==
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The behaviour of the d(GGTATACC) oligonucleotide has been investigated by, X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa, ( approximately 20 000 atm). Four 3D-structures of the A-DNA form (at, ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A, resolution. In addition to the diffraction pattern of the A-form, the, broad meridional streaks previously explained by occluded B-DNA octamers, within the channels of the crystalline A-form matrix were observed up to, at least 2 GPa. This work highlights an important property of nucleic, acids, their capability to withstand very high pressures, while keeping in, such conditions a nearly invariant geometry of base pairs that store and, carry genetic information. The double-helix base-paired architecture, behaves as a molecular spring, which makes it especially adapted to very, harsh conditions. These features may have contributed to the emergence of, a RNA World at prebiotic stage.
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The behaviour of the d(GGTATACC) oligonucleotide has been investigated by X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa (approximately 20,000 atm). Four 3D-structures of the A-DNA form (at ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A resolution. In addition to the diffraction pattern of the A-form, the broad meridional streaks previously explained by occluded B-DNA octamers within the channels of the crystalline A-form matrix were observed up to at least 2 GPa. This work highlights an important property of nucleic acids, their capability to withstand very high pressures, while keeping in such conditions a nearly invariant geometry of base pairs that store and carry genetic information. The double-helix base-paired architecture behaves as a molecular spring, which makes it especially adapted to very harsh conditions. These features may have contributed to the emergence of a RNA World at prebiotic stage.
==About this Structure==
==About this Structure==
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2PLB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Spm Binding Site For Residue B 20'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLB OCA].
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2PLB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Spm+Binding+Site+For+Residue+B+20'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLB OCA].
==Reference==
==Reference==
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Adaptation of the base-paired double-helix molecular architecture to extreme pressure., Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R, Nucleic Acids Res. 2007 Jul 7;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17617642 17617642]
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Adaptation of the base-paired double-helix molecular architecture to extreme pressure., Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R, Nucleic Acids Res. 2007;35(14):4800-8. Epub 2007 Jul 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17617642 17617642]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Fourme, R.]]
[[Category: Fourme, R.]]
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[[Category: high-pressure]]
[[Category: high-pressure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 10:42:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:30:39 2008''

Revision as of 16:30, 21 February 2008

Image:2plb.gif

2plb, resolution 1.60Å

Drag the structure with the mouse to rotate

D(GTATACC) under hydrostatic pressure of 1.39 GPa

Overview

The behaviour of the d(GGTATACC) oligonucleotide has been investigated by X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa (approximately 20,000 atm). Four 3D-structures of the A-DNA form (at ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A resolution. In addition to the diffraction pattern of the A-form, the broad meridional streaks previously explained by occluded B-DNA octamers within the channels of the crystalline A-form matrix were observed up to at least 2 GPa. This work highlights an important property of nucleic acids, their capability to withstand very high pressures, while keeping in such conditions a nearly invariant geometry of base pairs that store and carry genetic information. The double-helix base-paired architecture behaves as a molecular spring, which makes it especially adapted to very harsh conditions. These features may have contributed to the emergence of a RNA World at prebiotic stage.

About this Structure

2PLB is a Protein complex structure of sequences from [1] with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Adaptation of the base-paired double-helix molecular architecture to extreme pressure., Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R, Nucleic Acids Res. 2007;35(14):4800-8. Epub 2007 Jul 7. PMID:17617642

Page seeded by OCA on Thu Feb 21 18:30:39 2008

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