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ATP-dependent DNA ligase from bacteriophage T7

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==Catalytic function with DNA==
==Catalytic function with DNA==
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ATP-dependent DNA ligase from bacteriophage T7 amends a fractured DNA strand through esterification of a 5'- phosphoryl to a 3'- hydroxyl group<ref name="Bacteriophage"/>. This mechanism occurs with the aid of ATP in several steps. First, the ligase is activated through a <scene name='56/567310/Amp_complex/1'>phosphoramidate bond with a lysine residue</scene> in the active site (Lys 34). A pyrophosphate leaves and the enzyme-AMP complex is formed. Next, the AMP is transferred to the 5' phosphate group at the nick in the DNA. Finally, T7 ligase creates the phosphodiester bond between the 5' -phosphoryl and the 3' – hydroxyl group, with AMP being freed<ref name="Crystal"/>. All ATP-dependent DNA ligases contain a conserved amino acid sequence of KxDGxR<ref name="Bacteriophage"/>. This includes the lysine residue which binds the ATP in the groove between the two domains.
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ATP-dependent DNA ligase from bacteriophage T7 amends a fractured DNA strand through esterification of a 5'- phosphoryl to a 3'- hydroxyl group<ref name="Bacteriophage"/>. This mechanism occurs with the aid of ATP in several steps. First, the ligase is activated through a <scene name='56/567310/Amp_complex/1'>phosphoramidate bond with a lysine residue</scene> in the active site (Lys 34). A pyrophosphate leaves and the enzyme-AMP complex is formed. Next, the AMP is transferred to the 5' phosphate group at the nick in the DNA. Finally, T7 ligase creates the phosphodiester bond between the 5' -phosphoryl and the 3' – hydroxyl group, with AMP being freed<ref name="Crystal"/>. All ATP-dependent DNA ligases contain a conserved amino acid sequence of <scene name='56/567310/Residues_34-39/1'>KxDGxR</scene><ref name="Bacteriophage"/>. This includes the lysine residue which binds the ATP in the groove between the two domains.
</StructureSection>
</StructureSection>

Revision as of 07:03, 7 November 2013

ATP-DEPENDENT DNA LIGASE FROM BACTERIOPHAGE T7

Structure of Bacteriophage T7 DNA Ligase(PDB entry 1a0i)

Drag the structure with the mouse to rotate


References

  1. ↑ McGrath S, van Sinderen D. Bacteriophage: Genetics and Molecular Biology. Norfolk: Caister Academic Press, 2007. Print.
  2. ↑ Berg, Jeremy M, Stryer, Lubert, Tymoczko, John L. Biochemistry. Sixth edition. New York: W.H. Freeman and Company, 2007: 796. Print.
  3. ↑ 3.0 3.1 3.2 Doherty AJ, Ashford SR, Subramanya HS, Wigley DB. Bacteriophage T7 DNA ligase. Overexpression, purification, crystallization, and characterization. J Biol Chem. 1996 May 10;271(19):11083-9. PMID:8626651
  4. ↑ 4.0 4.1 4.2 4.3 4.4 Subramanya HS, Doherty AJ, Ashford SR, Wigley DB. Crystal structure of an ATP-dependent DNA ligase from bacteriophage T7. Cell. 1996 May 17;85(4):607-15. PMID:8653795

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