3j0d
From Proteopedia
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| - | [[Image:3j0d.png|left|200px]] | ||
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{{STRUCTURE_3j0d| PDB=3j0d | SCENE= }} | {{STRUCTURE_3j0d| PDB=3j0d | SCENE= }} | ||
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===Models for the T. thermophilus ribosome recycling factor bound to the E. coli post-termination complex=== | ===Models for the T. thermophilus ribosome recycling factor bound to the E. coli post-termination complex=== | ||
| + | {{ABSTRACT_PUBMED_22388519}} | ||
| - | + | ==Function== | |
| + | [[http://www.uniprot.org/uniprot/RRF_THET8 RRF_THET8]] Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity). [[http://www.uniprot.org/uniprot/RL11_ECOLI RL11_ECOLI]] This protein binds directly to 23S ribosomal RNA. Forms the L11 stalk, which is mobile in the ribosome, indicating its contribution to the activity of initiation, elongation and release factors.[HAMAP-Rule:MF_00736_B] [[http://www.uniprot.org/uniprot/RS12_ECOLI RS12_ECOLI]] With S4 and S5 plays an important role in translational accuracy.[HAMAP-Rule:MF_00403_B] Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).[HAMAP-Rule:MF_00403_B] Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit.[HAMAP-Rule:MF_00403_B] | ||
==About this Structure== | ==About this Structure== | ||
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==See Also== | ==See Also== | ||
| + | *[[Ribosomal protein L11|Ribosomal protein L11]] | ||
*[[Ribosomal protein S12|Ribosomal protein S12]] | *[[Ribosomal protein S12|Ribosomal protein S12]] | ||
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID:022388519</ref><references group="xtra"/> | + | <ref group="xtra">PMID:022388519</ref><references group="xtra"/><references/> |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
Revision as of 06:15, 13 November 2013
Contents |
Models for the T. thermophilus ribosome recycling factor bound to the E. coli post-termination complex
Template:ABSTRACT PUBMED 22388519
Function
[RRF_THET8] Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity). [RL11_ECOLI] This protein binds directly to 23S ribosomal RNA. Forms the L11 stalk, which is mobile in the ribosome, indicating its contribution to the activity of initiation, elongation and release factors.[HAMAP-Rule:MF_00736_B] [RS12_ECOLI] With S4 and S5 plays an important role in translational accuracy.[HAMAP-Rule:MF_00403_B] Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).[HAMAP-Rule:MF_00403_B] Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit.[HAMAP-Rule:MF_00403_B]
About this Structure
3j0d is a 11 chain structure with sequence from Escherichia coli and Thermus thermophilus. Full crystallographic information is available from OCA.
See Also
Reference
- Yokoyama T, Shaikh TR, Iwakura N, Kaji H, Kaji A, Agrawal RK. Structural insights into initial and intermediate steps of the ribosome-recycling process. EMBO J. 2012 Mar 2;31(7):1836-46. doi: 10.1038/emboj.2012.22. PMID:22388519 doi:10.1038/emboj.2012.22
