2pxc
From Proteopedia
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==Overview== | ==Overview== | ||
| - | We have determined the high resolution crystal structure of the | + | We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Alderton, D.]] | [[Category: Alderton, D.]] | ||
[[Category: Assenberg, R.]] | [[Category: Assenberg, R.]] | ||
| - | [[Category: Fuller, S | + | [[Category: Fuller, S D.]] |
| - | [[Category: Grimes, J | + | [[Category: Grimes, J M.]] |
| - | [[Category: Hurrelbrink, R | + | [[Category: Hurrelbrink, R J.]] |
| - | [[Category: OPPF, Oxford | + | [[Category: OPPF, Oxford Protein Production Facility.]] |
| - | [[Category: Owens, R | + | [[Category: Owens, R J.]] |
[[Category: Ren, J.]] | [[Category: Ren, J.]] | ||
| - | [[Category: Stuart, D | + | [[Category: Stuart, D I.]] |
[[Category: Verma, A.]] | [[Category: Verma, A.]] | ||
| - | [[Category: Walter, T | + | [[Category: Walter, T S.]] |
[[Category: G3A]] | [[Category: G3A]] | ||
[[Category: SAM]] | [[Category: SAM]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:34:04 2008'' |
Revision as of 16:34, 21 February 2008
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Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAM and GTPA
Overview
We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.
About this Structure
2PXC is a Single protein structure of sequence from Murray valley encephalitis virus with and as ligands. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Full crystallographic information is available from OCA.
Reference
Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues., Assenberg R, Ren J, Verma A, Walter TS, Alderton D, Hurrelbrink RJ, Fuller SD, Bressanelli S, Owens RJ, Stuart DI, Grimes JM, J Gen Virol. 2007 Aug;88(Pt 8):2228-36. PMID:17622627
Page seeded by OCA on Thu Feb 21 18:34:04 2008
Categories: Murray valley encephalitis virus | RNA-directed RNA polymerase | Single protein | Alderton, D. | Assenberg, R. | Fuller, S D. | Grimes, J M. | Hurrelbrink, R J. | OPPF, Oxford Protein Production Facility. | Owens, R J. | Ren, J. | Stuart, D I. | Verma, A. | Walter, T S. | G3A | SAM | G3a | Gtpa | Methyltransferase | Oppf | Oxford protein production facility | Sam | Structural genomics
