Sandbox Reserved 779

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	My protein is beta-lactoglobulin.		My protein is beta-lactoglobulin.
-	β-Lactoglobulin is the major whey protein of cow and sheep's milk	+	β-Lac
		+
		+	1. Introduction
		+
		+	Function:Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
		+
		+	relevant background:
		+
		+	class of protein :Belongs to the calycin superfamily. Lipocalin family.
		+	overall function of Lipocalin family: The lipocalins are a family of proteins which transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids.
		+	Lipocalins have been associated with many biological processes, among them immune response, pheromone transport, biological prostaglandin synthesis, retinoid binding, and cancer cell interactions.
		+
		+	short description of protein fold: They share limited regions of sequence homology and a common tertiary structure architecture.[2][3][4][5][6] This is an eight stranded antiparallel beta-barrel with a repeated + 1 topology enclosing an internal ligand binding site.[5][4]
		+
		+	organisms:These proteins are found in gram negative bacteria, vertebrate cells, and invertebrate cells, and in plants.
		+
		+	other information: Subunit structure : Under physiological conditions beta-lactoglobulin exists as an equilibrium mixture of monomeric and dimeric forms.
		+	Subcellular location: Secreted.
		+	Tissue specificity: Synthesized in mammary gland and secreted in milk.
		+	Post-translational modification : Alternate disulfide bonds occur in equal amounts in all variants examined.
		+	Allergenic properties:Causes an allergic reaction in human. Is one of the causes of cow's milk allergy.
		+	Miscellaneous The B variant sequence is shown.
		+
		+	2. Structure
		+
		+	overall description of the structure of the protein:
		+	a. oligomeric state
		+	b. description of secondary structure
		+	c. description of active residues of the protein and where they are on the protein
		+	d. description of any ligands in the structure
		+	e. methods used to solve the structure : X-ray crystallography, NMR, EM
		+
		+	upload the structure (number code: 2Q2M)
		+	secondary structure elements:
		+	protein fold and how thats important for the function
		+	ligands if theres ligands
		+	the active site if relevant
		+	features of protein that are important for function
		+	zoom in on the active site, label the important active site residues, and hughlight those residues in a different color (make it look pretty)
		+
		+	3. mechanism of action
		+	how the protein function. include chemical structure of any relevant ligands, inhibitors, or important states in the reaction pathway.
		+
		+	4. implications or possible application
		+	describe any uses or application that have been made of the protein
		+
		+	5. references
		+	at least 5

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Revision as of 20:05, 18 November 2013

This Sandbox is Reserved from Sep 25, 2013, through Mar 31, 2014 for use in the course "BCH455/555 Proteins and Molecular Mechanisms" taught by Michael B. Goshe at the North Carolina State University. This reservation includes Sandbox Reserved 299, Sandbox Reserved 300 and Sandbox Reserved 760 through Sandbox Reserved 779.

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My protein is beta-lactoglobulin.

β-Lac

1. Introduction

Function:Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.

relevant background:

class of protein :Belongs to the calycin superfamily. Lipocalin family. overall function of Lipocalin family: The lipocalins are a family of proteins which transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids. Lipocalins have been associated with many biological processes, among them immune response, pheromone transport, biological prostaglandin synthesis, retinoid binding, and cancer cell interactions.

short description of protein fold: They share limited regions of sequence homology and a common tertiary structure architecture.[2][3][4][5][6] This is an eight stranded antiparallel beta-barrel with a repeated + 1 topology enclosing an internal ligand binding site.[5][4]

organisms:These proteins are found in gram negative bacteria, vertebrate cells, and invertebrate cells, and in plants.

other information: Subunit structure : Under physiological conditions beta-lactoglobulin exists as an equilibrium mixture of monomeric and dimeric forms. Subcellular location: Secreted. Tissue specificity: Synthesized in mammary gland and secreted in milk. Post-translational modification : Alternate disulfide bonds occur in equal amounts in all variants examined. Allergenic properties:Causes an allergic reaction in human. Is one of the causes of cow's milk allergy. Miscellaneous The B variant sequence is shown.

2. Structure

overall description of the structure of the protein: a. oligomeric state b. description of secondary structure c. description of active residues of the protein and where they are on the protein d. description of any ligands in the structure e. methods used to solve the structure : X-ray crystallography, NMR, EM

upload the structure (number code: 2Q2M) secondary structure elements: protein fold and how thats important for the function ligands if theres ligands the active site if relevant features of protein that are important for function zoom in on the active site, label the important active site residues, and hughlight those residues in a different color (make it look pretty)

3. mechanism of action how the protein function. include chemical structure of any relevant ligands, inhibitors, or important states in the reaction pathway.

4. implications or possible application describe any uses or application that have been made of the protein

5. references at least 5