2q6t
From Proteopedia
(New page: 200px<br /><applet load="2q6t" size="350" color="white" frame="true" align="right" spinBox="true" caption="2q6t, resolution 2.90Å" /> '''Crystal structure of...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The ring-shaped hexameric DnaB helicase unwinds duplex DNA at the | + | The ring-shaped hexameric DnaB helicase unwinds duplex DNA at the replication fork of eubacteria. We have solved the crystal structure of the full-length Thermus aquaticus DnaB monomer, or possibly dimer, at 2.9 A resolution. DnaB is a highly flexible two domain protein. The C-terminal domain exhibits a RecA-like core fold and contains all the conserved sequence motifs that are characteristic of the DnaB helicase family. The N-terminal domain contains an additional helical hairpin that makes it larger than previously appreciated. Several DnaB mutations that modulate its interaction with primase are found in this hairpin. The similarity in the fold of the DnaB N-terminal domain with that of the C-terminal helicase-binding domain (HBD) of the DnaG primase also includes this hairpin. Comparison of hexameric homology models of DnaB with the structure of the papillomavirus E1 helicase suggests the two helicases may function through different mechanisms despite their sharing a common ancestor. |
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
| - | The crystal structure of the Thermus aquaticus DnaB helicase monomer., Bailey S, Eliason WK, Steitz TA, Nucleic Acids Res. 2007 Jul 1 | + | The crystal structure of the Thermus aquaticus DnaB helicase monomer., Bailey S, Eliason WK, Steitz TA, Nucleic Acids Res. 2007;35(14):4728-36. Epub 2007 Jul 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17606462 17606462] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermus aquaticus]] | [[Category: Thermus aquaticus]] | ||
[[Category: Bailey, S.]] | [[Category: Bailey, S.]] | ||
| - | [[Category: Eliason, W | + | [[Category: Eliason, W K.]] |
| - | [[Category: Steitz, T | + | [[Category: Steitz, T A.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: dnab]] | [[Category: dnab]] | ||
| Line 21: | Line 21: | ||
[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:36:37 2008'' |
Revision as of 16:36, 21 February 2008
|
Crystal structure of the Thermus aquaticus DnaB monomer
Overview
The ring-shaped hexameric DnaB helicase unwinds duplex DNA at the replication fork of eubacteria. We have solved the crystal structure of the full-length Thermus aquaticus DnaB monomer, or possibly dimer, at 2.9 A resolution. DnaB is a highly flexible two domain protein. The C-terminal domain exhibits a RecA-like core fold and contains all the conserved sequence motifs that are characteristic of the DnaB helicase family. The N-terminal domain contains an additional helical hairpin that makes it larger than previously appreciated. Several DnaB mutations that modulate its interaction with primase are found in this hairpin. The similarity in the fold of the DnaB N-terminal domain with that of the C-terminal helicase-binding domain (HBD) of the DnaG primase also includes this hairpin. Comparison of hexameric homology models of DnaB with the structure of the papillomavirus E1 helicase suggests the two helicases may function through different mechanisms despite their sharing a common ancestor.
About this Structure
2Q6T is a Single protein structure of sequence from Thermus aquaticus with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of the Thermus aquaticus DnaB helicase monomer., Bailey S, Eliason WK, Steitz TA, Nucleic Acids Res. 2007;35(14):4728-36. Epub 2007 Jul 1. PMID:17606462
Page seeded by OCA on Thu Feb 21 18:36:37 2008
Categories: Single protein | Thermus aquaticus | Bailey, S. | Eliason, W K. | Steitz, T A. | SO4 | Dnab | Helicase | Replication
