2q73
From Proteopedia
(New page: 200px<br /><applet load="2q73" size="350" color="white" frame="true" align="right" spinBox="true" caption="2q73, resolution 1.80Å" /> '''Crystal structure of...) |
|||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | Mobile gene cassettes collectively contain a highly diverse pool of novel | + | Mobile gene cassettes collectively contain a highly diverse pool of novel genes that encode many novel adaptive functions. In the non-clinical context, the function of almost all of the encoded proteins remains unknown despite the enormous size of this mobile gene pool. We have been characterizing cassette arrays by taking advantage of the fact that they cluster at discrete sites in chromosomes; even large arrays are thus recoverable in a relatively small number of clones in genomic libraries. In one assembled array of 116 cassettes from the marine bacterium Vibrio sp. DAT722, a putative MazG protein is encoded within the 21st cassette. Because MazG proteins are implicated in a number of cellular processes, including house-cleaning and stress survival, the presence of such a protein in a mobile cassette was noteworthy. Here we solve the crystal structure of this alpha-helical protein, and define both open and closed states of a new variant of the MazG family. Functional assays confirm that the protein is a dNTP pyrophosphohydrolase, with marked preferences for dCTP and dATP. We hypothesize that iMazG acts as a house-cleaning enzyme, preventing the incorporation of damaging non-canonical nucleotides into host-cell DNA. |
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
| - | A putative house-cleaning enzyme encoded within an integron array: 1.8 A crystal structure defines a new MazG subtype., Robinson A, Guilfoyle AP, Harrop SJ, Boucher Y, Stokes HW, Curmi PM, Mabbutt BC, Mol Microbiol. 2007 Sep 24 | + | A putative house-cleaning enzyme encoded within an integron array: 1.8 A crystal structure defines a new MazG subtype., Robinson A, Guilfoyle AP, Harrop SJ, Boucher Y, Stokes HW, Curmi PM, Mabbutt BC, Mol Microbiol. 2007 Nov;66(3):610-21. Epub 2007 Sep 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17892463 17892463] |
[[Category: Nucleoside-triphosphate diphosphatase]] | [[Category: Nucleoside-triphosphate diphosphatase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vibrio sp. dat722]] | [[Category: Vibrio sp. dat722]] | ||
[[Category: Boucher, Y.]] | [[Category: Boucher, Y.]] | ||
| - | [[Category: Curmi, P | + | [[Category: Curmi, P M.G.]] |
| - | [[Category: Guilfoyle, A | + | [[Category: Guilfoyle, A P.]] |
| - | [[Category: Harrop, S | + | [[Category: Harrop, S J.]] |
| - | [[Category: Mabbutt, B | + | [[Category: Mabbutt, B C.]] |
[[Category: Robinson, A.]] | [[Category: Robinson, A.]] | ||
| - | [[Category: Stokes, H | + | [[Category: Stokes, H W.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| Line 27: | Line 27: | ||
[[Category: vibrio]] | [[Category: vibrio]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:36:51 2008'' |
Revision as of 16:36, 21 February 2008
|
Crystal structure of iMazG from Vibrio DAT 722: Ctag-iMazG (P41212)
Overview
Mobile gene cassettes collectively contain a highly diverse pool of novel genes that encode many novel adaptive functions. In the non-clinical context, the function of almost all of the encoded proteins remains unknown despite the enormous size of this mobile gene pool. We have been characterizing cassette arrays by taking advantage of the fact that they cluster at discrete sites in chromosomes; even large arrays are thus recoverable in a relatively small number of clones in genomic libraries. In one assembled array of 116 cassettes from the marine bacterium Vibrio sp. DAT722, a putative MazG protein is encoded within the 21st cassette. Because MazG proteins are implicated in a number of cellular processes, including house-cleaning and stress survival, the presence of such a protein in a mobile cassette was noteworthy. Here we solve the crystal structure of this alpha-helical protein, and define both open and closed states of a new variant of the MazG family. Functional assays confirm that the protein is a dNTP pyrophosphohydrolase, with marked preferences for dCTP and dATP. We hypothesize that iMazG acts as a house-cleaning enzyme, preventing the incorporation of damaging non-canonical nucleotides into host-cell DNA.
About this Structure
2Q73 is a Single protein structure of sequence from Vibrio sp. dat722 with as ligand. Active as Nucleoside-triphosphate diphosphatase, with EC number 3.6.1.19 Full crystallographic information is available from OCA.
Reference
A putative house-cleaning enzyme encoded within an integron array: 1.8 A crystal structure defines a new MazG subtype., Robinson A, Guilfoyle AP, Harrop SJ, Boucher Y, Stokes HW, Curmi PM, Mabbutt BC, Mol Microbiol. 2007 Nov;66(3):610-21. Epub 2007 Sep 24. PMID:17892463
Page seeded by OCA on Thu Feb 21 18:36:51 2008
