Glutamate receptor (GluA2)

Overview

The homomeric rat GluA2 receptor arranged in a 'Y'-shape with the ^[5]. This structure is a functional homotetramer of the AMPA-subtype; native ionotropic glutamate receptors are almost exclusively heterotetramers. .

Domains

The subunits themselves are modular ^[7]and the major domains are found in layers in the tetrameric structure.

• The 'top' layer is composed of the

This .

• participates directly in agonist/competitive antagonist binding, affects activation gating, and is the portion that forms the 'middle' layer.

in the structure.

The small molecule ^[8], was studied as a treatment for stroke because it had demonstrated neuroprotective efficacy in experimental models of stroke and tolerability in healthy volunteers; however, in a multicenter, double-blind, randomized, placebo-controlled phase II trial, it was found to have significant sedative effects in patients with acute stroke which precludes its further development as a neuroprotective agent^[9].

• is the portion that forms the membrane-spanning on the 'bottom' of the solved structure.

To help give a better idea of how the glutamate receptor is oriented on the cell surface in the membrane lipid bilayer, as calculated by the Orientations of Proteins in Membranes database (University of Michigan, USA) is shown with the red patch of spheres indicating the boundary of the hydrophobic core closet to the outside of the cell and the dark blue patch of spheres indicating the boundary closest to the inside of the cell.

• The carboxy-terminal domain that plays a role in both receptor localization and regulation is not seen in the structure but would be below the transmembrane domain as it is cytoplasmic.

Domain swapping between the subunits and symmetry mismatch between the domains

• Unanticipated is the domain swapping and crossover that occurs between the subunits interactions. In order to discuss the remarkable swapping, it is best to :
      A       B       C        D

• Considering each chain, there is crossover as the pairs of subunits seen in the ATD are swapped in the LBD.

In the ATD domain -

• .
• And the .
• While that is going on, in the ATD there is also inter-pair interactions mediated between . Note this view really highlights the two-fold symmetry between the A-B and C-D pairs at the level of the ATD.

In the LBD domain -

• Whereas in the ATD domain A and B paired up, in the LBD.
• And the .
• While that is going on, in the LBD there is also extensive inter-pair interactions mediated between . Note this view highlights the two-fold symmetry between the A-D and B-C pairs at the level of the LBD. .

The domain swapping can be observed from the side following the backbone of each chain as well: , , , and . And .

• As explored further in a later section below , the . Thus, remarkably, the symmetry switches from an overall two-fold symmetry for the ATD and LBD to four-fold for the TMD.

Subunit non-equivalence

As a result of the swapping and symmetry mismatch, there is subunit non-equivalence; even though all the chains are the same chemically, there are two distinct conformations of the subunits. This means there are two matching pairs of subunits. The pairs are illustrated below and the morphs referred to below will show in the main window on the left:

• Subunit A is equivalent to Subunit C (in the small structure window in this section). In the main window, a .
• Subunit B is equivalent to Subunit D (in the small structure window in this section). In the main window, a .

However, each of the subunit A/C group though is distinct from those of the B/D group. Having established the two equivalent groups we can simplify the discussion of the relationship between the two pairs by focusing solely on comparing Subunit A' and Subunit B.
The domains themselves stay relatively static between the two conformational forms, with the linkers in between and the resulting arrangement changing. This is best illustrated by superposition of the individual domains of Subunit A and Subunit B:

• .
• .
• .

between the two conformational forms.

The linkers are key; besides playing roles in domain swapping and resolving the symmetry mismatch, they are also responsible for relaying the modulation signals from the ATD to the other domains and signaling the conformational change of the LBD to control the opening and closing of the pore. Beyond the two conformations seen here though this particular structure (3kg2) of the receptor does not shed light on the transduction process.

Transmembrane domain architecture and the occluded pore

• M1
• M2
• M3
• M4

• The segments shown again, .

There is consistent with the channel being in a closed state with the antagonist (ZK200775) bound to the LBD.

It is that occludes the channel. [BE PATIENT as a small surface is generated.]

Note . This is in part is why the symmetry is only approximately four-fold and is one of the several intriguing observations in regard to symmetry for this macromolecule. In fact, the location of two-fold symmetry at the ends of M3 is just above the portion that spans the membrane and is close to the last region of the structure that doesn't show four-fold symmetry as abruptly below this point everything is four-fold symmetric.

• To better observe the contributions of each of the membrane segments to the subunit-subunit interactions, . [Note: this scene generates a substantial surface which may take about a minute to calculate. Be patient.]

Note that the M4 segment associates with the ion-channel core of an adjacent subunit.

.

• The TMD domain of the GluA2 receptor shares structural and sequence similarity with the pore region of the potassium (K+), as hinted at by earlier work^[10][11][12]. Here the pore region of Streptomyces lividans potassium channel (1bl8), specifically the . The of the K+ channel even though these portions weren't even included in the calculation of the alignment seen here.