2qh2

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(New page: 200px<br /><applet load="2qh2" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qh2" /> '''Solution structure of the CR7 terminal hairp...)
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==Overview==
==Overview==
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The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains, required for 3' end processing, localization, and accumulation. The, terminal loop of the CR7 domain contains the CAB box (ugAG) required for, targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence, required for accumulation and processing. To dissect out the contributions, of the CR7 stem loop to hTR processing and localization, we solved the, solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA, snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These, structures, together with analysis of localization, processing, and, accumulation of hTRs containing nucleotide substitutions in the CR7, domain, identified the sequence and structural requirements of the hTR, processing and CB localization signals and showed that these signals are, functionally independent. Further, 3' end processing was found to be a, prerequisite for translocation of hTR to CBs.
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The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.
==About this Structure==
==About this Structure==
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Structural and functional characterization of human telomerase RNA processing and cajal body localization signals., Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J, Mol Cell. 2007 Sep 21;27(6):869-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17889661 17889661]
Structural and functional characterization of human telomerase RNA processing and cajal body localization signals., Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J, Mol Cell. 2007 Sep 21;27(6):869-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17889661 17889661]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Breece, K.E.]]
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[[Category: Breece, K E.]]
[[Category: Chim, N]]
[[Category: Chim, N]]
[[Category: Feigon, J.]]
[[Category: Feigon, J.]]
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[[Category: Theimer, C.A.]]
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[[Category: Theimer, C A.]]
[[Category: loop]]
[[Category: loop]]
[[Category: rna]]
[[Category: rna]]
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[[Category: wobble base pair]]
[[Category: wobble base pair]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:34:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:39:26 2008''

Revision as of 16:39, 21 February 2008

Image:2qh2.gif

2qh2

Drag the structure with the mouse to rotate

Solution structure of the CR7 terminal hairpin loop from human telomerase RNA

Overview

The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.

About this Structure

2QH2 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structural and functional characterization of human telomerase RNA processing and cajal body localization signals., Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J, Mol Cell. 2007 Sep 21;27(6):869-81. PMID:17889661

Page seeded by OCA on Thu Feb 21 18:39:26 2008

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