2qij
From Proteopedia
(New page: 200px<br /><applet load="2qij" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qij, resolution 8.9Å" /> '''Hepatitis B Capsid Pr...) |
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==Overview== | ==Overview== | ||
| - | Hepatitis B core (HBc) particles have been extensively exploited as | + | Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20,000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 A resolution and data were collected to 99.6% completeness at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Hepatitis b virus subtype adw]] | [[Category: Hepatitis b virus subtype adw]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ho, K | + | [[Category: Ho, K L.]] |
| - | [[Category: McNae, I | + | [[Category: McNae, I W.]] |
| - | [[Category: Tan, W | + | [[Category: Tan, W S]] |
| - | [[Category: Walkinshaw, M | + | [[Category: Walkinshaw, M D.]] |
[[Category: icosahedral virus]] | [[Category: icosahedral virus]] | ||
[[Category: viral capsid protein]] | [[Category: viral capsid protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:39:42 2008'' |
Revision as of 16:39, 21 February 2008
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Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.
Overview
Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20,000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 A resolution and data were collected to 99.6% completeness at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells.
About this Structure
2QIJ is a Single protein structure of sequence from Hepatitis b virus subtype adw. Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension., Tan WS, McNae IW, Ho KL, Walkinshaw MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt, 8):642-7. Epub 2007 Jul 21. PMID:17671358
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