2qju

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(New page: 200px<br /><applet load="2qju" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qju, resolution 2.90&Aring;" /> '''Crystal Structure of...)
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==Overview==
==Overview==
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Tricyclic antidepressants exert their pharmacological effect-inhibiting, the reuptake of serotonin, norepinephrine, and dopamine-by directly, blocking neurotransmitter transporters (SERT, NET, and DAT, respectively), in the presynaptic membrane. The drug-binding site and the mechanism of, this inhibition are poorly understood. We determined the crystal structure, at 2.9 A of the bacterial leucine transporter (LeuT), a homolog of SERT, NET, and DAT, in complex with leucine and the antidepressant desipramine., Desipramine binds at the inner end of the extracellular cavity of the, transporter and is held in place by a hairpin loop and by a salt bridge., This binding site is separated from the leucine-binding site by the, extracellular gate of the transporter. By directly locking the gate, desipramine prevents conformational changes and blocks substrate, transport. Mutagenesis experiments on human SERT and DAT indicate that, both the desipramine-binding site and its inhibition mechanism are, probably conserved in the human neurotransmitter transporters.
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Tricyclic antidepressants exert their pharmacological effect-inhibiting the reuptake of serotonin, norepinephrine, and dopamine-by directly blocking neurotransmitter transporters (SERT, NET, and DAT, respectively) in the presynaptic membrane. The drug-binding site and the mechanism of this inhibition are poorly understood. We determined the crystal structure at 2.9 angstroms of the bacterial leucine transporter (LeuT), a homolog of SERT, NET, and DAT, in complex with leucine and the antidepressant desipramine. Desipramine binds at the inner end of the extracellular cavity of the transporter and is held in place by a hairpin loop and by a salt bridge. This binding site is separated from the leucine-binding site by the extracellular gate of the transporter. By directly locking the gate, desipramine prevents conformational changes and blocks substrate transport. Mutagenesis experiments on human SERT and DAT indicate that both the desipramine-binding site and its inhibition mechanism are probably conserved in the human neurotransmitter transporters.
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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LeuT-Desipramine Structure Reveals How Antidepressants Block Neurotransmitter Reuptake., Zhou Z, Zhen J, Karpowich NK, Goetz RM, Law CJ, Reith ME, Wang DN, Science. 2007 Aug 9;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17690258 17690258]
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LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake., Zhou Z, Zhen J, Karpowich NK, Goetz RM, Law CJ, Reith ME, Wang DN, Science. 2007 Sep 7;317(5843):1390-3. Epub 2007 Aug 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17690258 17690258]
[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Karpowich, N.K.]]
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[[Category: Karpowich, N K.]]
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[[Category: Wang, D.N.]]
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[[Category: Wang, D N.]]
[[Category: Zhou, Z.]]
[[Category: Zhou, Z.]]
[[Category: BOG]]
[[Category: BOG]]
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[[Category: transport protein]]
[[Category: transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:34:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:39:52 2008''

Revision as of 16:39, 21 February 2008

Image:2qju.jpg

2qju, resolution 2.90Å

Drag the structure with the mouse to rotate

Crystal Structure of an NSS Homolog with Bound Antidepressant

Overview

Tricyclic antidepressants exert their pharmacological effect-inhibiting the reuptake of serotonin, norepinephrine, and dopamine-by directly blocking neurotransmitter transporters (SERT, NET, and DAT, respectively) in the presynaptic membrane. The drug-binding site and the mechanism of this inhibition are poorly understood. We determined the crystal structure at 2.9 angstroms of the bacterial leucine transporter (LeuT), a homolog of SERT, NET, and DAT, in complex with leucine and the antidepressant desipramine. Desipramine binds at the inner end of the extracellular cavity of the transporter and is held in place by a hairpin loop and by a salt bridge. This binding site is separated from the leucine-binding site by the extracellular gate of the transporter. By directly locking the gate, desipramine prevents conformational changes and blocks substrate transport. Mutagenesis experiments on human SERT and DAT indicate that both the desipramine-binding site and its inhibition mechanism are probably conserved in the human neurotransmitter transporters.

About this Structure

2QJU is a Single protein structure of sequence from Aquifex aeolicus with , , , and as ligands. Full crystallographic information is available from OCA.

Reference

LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake., Zhou Z, Zhen J, Karpowich NK, Goetz RM, Law CJ, Reith ME, Wang DN, Science. 2007 Sep 7;317(5843):1390-3. Epub 2007 Aug 9. PMID:17690258

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