1ygw

From Proteopedia

Revision as of 14:28, 30 October 2007

NMR STRUCTURE OF RIBONUCLEASE T1, 34 STRUCTURES

Overview

Limits of NMR structure determination using multidimensional NMR, spectroscopy, variable target function calculations and relaxation matrix, analysis were explored using the model protein ribonuclease T1 (RNase T1)., The enzyme consists of 104 amino acid residues and has a molecular mass of, approximately 11 kDa. Primary experimental data comprise 1856 assigned NOE, intensities, 493 3J coupling constants and 62 values of amid proton, exchange rates. From these data, 2580 distance bounds, 168 allowed ranges, for torsional angles and stereospecific assignments for 75% of, beta-methylene protons as well as for 80% of diastereotopic methyl groups, were derived. Whenever possible, the distance restraints were refined in a, relaxation matrix analysis including amid proton exchange data for, ... [(full description)]

About this Structure

1YGW is a [Single protein] structure of sequence from [Aspergillus oryzae]. Active as [Ribonuclease T(1)], with EC number [3.1.27.3]. Structure known Active Sites: CAT and REC. Full crystallographic information is available from [OCA].

Reference

Limits of NMR structure determination using variable target function calculations: ribonuclease T1, a case study., Pfeiffer S, Karimi-Nejad Y, Ruterjans H, J Mol Biol. 1997 Feb 21;266(2):400-23. PMID:9047372

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