2qox
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | The widely used antibiotic spectinomycin inhibits bacterial protein | + | The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle. |
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: Bacteria]] | [[Category: Bacteria]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Borovinskaya, M | + | [[Category: Borovinskaya, M A.]] |
| - | [[Category: Cate, J | + | [[Category: Cate, J H.D.]] |
[[Category: Fredrick, K.]] | [[Category: Fredrick, K.]] | ||
| - | [[Category: Holton, J | + | [[Category: Holton, J M.]] |
[[Category: Shoji, S.]] | [[Category: Shoji, S.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| Line 23: | Line 23: | ||
[[Category: rna-protein complex]] | [[Category: rna-protein complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:41:07 2008'' |
Revision as of 16:41, 21 February 2008
|
Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 50S subunit of the second 70S ribosome. The entire crystal structure contains two 70S ribosomes.
Overview
The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.
About this Structure
2QOX is a Protein complex structure of sequences from Bacteria with and as ligands. Full crystallographic information is available from OCA.
Reference
A steric block in translation caused by the antibiotic spectinomycin., Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH, ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:17696316
Page seeded by OCA on Thu Feb 21 18:41:07 2008
Categories: Bacteria | Protein complex | Borovinskaya, M A. | Cate, J H.D. | Fredrick, K. | Holton, J M. | Shoji, S. | MG | ZN | Ribosome | Rna-protein complex
