2qub

Lipase LipA from Serratia marcescens is a 613-amino acid enzyme belonging, to family I.3 of lipolytic enzymes that has an important biotechnological, application in the production of a chiral precursor for the coronary, vasodilator diltiazem. Like other family I.3 lipases, LipA is secreted by, Gram-negative bacteria via a type I secretion system and possesses 13, copies of a calcium binding tandem repeat motif, GGXGXDXUX (U, hydrophobic, amino acids), in the C-terminal part of the polypeptide chain. The 1.8-A, crystal structure of LipA reveals a close relation to eukaryotic lipases, whereas family I.1 and I.2 enzymes appear to be more distantly related., Interestingly, the structure shows for the N-terminal lipase domain a, variation on the canonical alpha/beta hydrolase fold in an open, conformation, where the putative lid helix is anchored by a Ca(2+) ion, essential for activity. Another novel feature observed in this lipase, structure is the presence of a helical hairpin additional to the putative, lid helix that exposes a hydrophobic surface to the aqueous medium and, might function as an additional lid. The tandem repeats form two separated, parallel beta-roll domains that pack tightly against each other., Variations of the consensus sequence of the tandem repeats within the, second beta-roll result in an asymmetric Ca(2+) binding on only one side, of the roll. The analysis of the properties of the beta-roll domains, suggests an intramolecular chaperone function.

2QUB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Known structural/functional Sites: <scene name='pdbsite=AC1:Ca Binding Site For Residue A 614'>AC1</scene>, <scene name='pdbsite=AC2:Ca Binding Site For Residue A 615'>AC2</scene>, <scene name='pdbsite=AC3:Ca Binding Site For Residue A 616'>AC3</scene>, <scene name='pdbsite=AC4:Ca Binding Site For Residue A 617'>AC4</scene>, <scene name='pdbsite=AC5:Ca Binding Site For Residue A 618'>AC5</scene>, <scene name='pdbsite=AC6:Ca Binding Site For Residue A 619'>AC6</scene>, <scene name='pdbsite=AC7:Ca Binding Site For Residue A 620'>AC7</scene>, <scene name='pdbsite=AC8:Ca Binding Site For Residue A 621'>AC8</scene>, <scene name='pdbsite=AC9:Ca Binding Site For Residue C 614'>AC9</scene>, <scene name='pdbsite=BC1:Ca Binding Site For Residue C 615'>BC1</scene>, <scene name='pdbsite=BC2:Ca Binding Site For Residue C 616'>BC2</scene>, <scene name='pdbsite=BC3:Ca Binding Site For Residue C 617'>BC3</scene>, <scene name='pdbsite=BC4:Ca Binding Site For Residue C 618'>BC4</scene>, <scene name='pdbsite=BC5:Ca Binding Site For Residue C 619'>BC5</scene>, <scene name='pdbsite=BC6:Ca Binding Site For Residue C 620'>BC6</scene>, <scene name='pdbsite=BC7:Ca Binding Site For Residue C 621'>BC7</scene>, <scene name='pdbsite=BC8:Ca Binding Site For Residue E 614'>BC8</scene>, <scene name='pdbsite=BC9:Ca Binding Site For Residue E 615'>BC9</scene>, <scene name='pdbsite=CC1:Ca Binding Site For Residue E 616'>CC1</scene>, <scene name='pdbsite=CC2:Ca Binding Site For Residue E 617'>CC2</scene>, <scene name='pdbsite=CC3:Ca Binding Site For Residue E 618'>CC3</scene>, <scene name='pdbsite=CC4:Ca Binding Site For Residue E 619'>CC4</scene>, <scene name='pdbsite=CC5:Ca Binding Site For Residue E 620'>CC5</scene>, <scene name='pdbsite=CC6:Ca Binding Site For Residue E 621'>CC6</scene>, <scene name='pdbsite=CC7:Ca Binding Site For Residue G 614'>CC7</scene>, <scene name='pdbsite=CC8:Ca Binding Site For Residue G 615'>CC8</scene>, <scene name='pdbsite=CC9:Ca Binding Site For Residue G 616'>CC9</scene>, <scene name='pdbsite=DC1:Ca Binding Site For Residue G 617'>DC1</scene>, <scene name='pdbsite=DC2:Ca Binding Site For Residue G 618'>DC2</scene>, <scene name='pdbsite=DC3:Ca Binding Site For Residue G 619'>DC3</scene>, <scene name='pdbsite=DC4:Ca Binding Site For Residue G 620'>DC4</scene>, <scene name='pdbsite=DC5:Ca Binding Site For Residue G 621'>DC5</scene>, <scene name='pdbsite=DC6:Ca Binding Site For Residue I 614'>DC6</scene>, <scene name='pdbsite=DC7:Ca Binding Site For Residue I 615'>DC7</scene>, <scene name='pdbsite=DC8:Ca Binding Site For Residue I 616'>DC8</scene>, <scene name='pdbsite=DC9:Ca Binding Site For Residue I 617'>DC9</scene>, <scene name='pdbsite=EC1:Ca Binding Site For Residue I 618'>EC1</scene>, <scene name='pdbsite=EC2:Ca Binding Site For Residue I 619'>EC2</scene>, <scene name='pdbsite=EC3:Ca Binding Site For Residue I 620'>EC3</scene>, <scene name='pdbsite=EC4:Ca Binding Site For Residue I 621'>EC4</scene>, <scene name='pdbsite=EC5:Ca Binding Site For Residue K 614'>EC5</scene>, <scene name='pdbsite=EC6:Ca Binding Site For Residue K 615'>EC6</scene>, <scene name='pdbsite=EC7:Ca Binding Site For Residue K 616'>EC7</scene>, <scene name='pdbsite=EC8:Ca Binding Site For Residue K 617'>EC8</scene>, <scene name='pdbsite=EC9:Ca Binding Site For Residue K 618'>EC9</scene>, <scene name='pdbsite=FC1:Ca Binding Site For Residue K 619'>FC1</scene>, <scene name='pdbsite=FC2:Ca Binding Site For Residue K 620'>FC2</scene> and <scene name='pdbsite=FC3:Ca Binding Site For Residue K 621'>FC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QUB OCA].

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