2qvw

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(New page: 200px<br /><applet load="2qvw" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qvw, resolution 3.000&Aring;" /> '''Structure of Giardi...)
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==Overview==
==Overview==
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The crystal structure of the enzyme Dicer from Giardia intestinalis was, solved to 3.3 A resolution by MAD using crystals belonging to space group, P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals, were derived from crystals that diffracted X-rays to 3.0 A resolution but, were refractory to structure determination because they were twinned. It, is shown here that the original Dicer crystals represent an unusual case, of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the, twinning problem was overcome, it was possible to calculate a, low-resolution electron-density map in space group P4(1) that was used to, build a partial molecular model. Experimental phases were sufficient to, identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided, the search for different crystallization conditions that yielded untwinned, crystals and ultimately a fully interpretable electron-density map.
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The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map.
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., Macrae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17704568 17704568]
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An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17704568 17704568]
[[Category: Giardia intestinalis]]
[[Category: Giardia intestinalis]]
[[Category: Ribonuclease III]]
[[Category: Ribonuclease III]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Doudna, J.A.]]
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[[Category: Doudna, J A.]]
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[[Category: MacRae, I.J]]
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[[Category: MacRae, I J]]
[[Category: MN]]
[[Category: MN]]
[[Category: dicer]]
[[Category: dicer]]
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[[Category: rnai]]
[[Category: rnai]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:30:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:42:48 2008''

Revision as of 16:42, 21 February 2008


2qvw, resolution 3.000Å

Drag the structure with the mouse to rotate

Structure of Giardia Dicer refined against twinned data

Overview

The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map.

About this Structure

2QVW is a Single protein structure of sequence from Giardia intestinalis with as ligand. Active as Ribonuclease III, with EC number 3.1.26.3 Full crystallographic information is available from OCA.

Reference

An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:17704568

Page seeded by OCA on Thu Feb 21 18:42:48 2008

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