4mn8

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'''Unreleased structure'''
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{{STRUCTURE_4mn8| PDB=4mn8 | SCENE= }}
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===Crystal structure of flg22 in complex with the FLS2 and BAK1 ectodomains===
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{{ABSTRACT_PUBMED_24114786}}
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The entry 4mn8 is ON HOLD until Paper Publication
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==Function==
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[[http://www.uniprot.org/uniprot/FLS2_ARATH FLS2_ARATH]] Constitutes the pattern-recognition receptor (PPR) that determines the specific perception of flagellin (flg22), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). Flagellin-binding to the receptor is the first step to initiate the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6), resulting in enhanced resistance against pathogens. Binding to the effector AvrPto1 from Pseudomonas syringae blocks the downstream plant immune response.<ref>PMID:10911994</ref> <ref>PMID:11340188</ref> <ref>PMID:11875555</ref> <ref>PMID:15085136</ref> <ref>PMID:19062288</ref> <ref>PMID:16377758</ref> [[http://www.uniprot.org/uniprot/BAK1_ARATH BAK1_ARATH]] Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Controls the expression of genes associated with innate immunity in the absence of pathogens or elicitors. Involved in brassinosteroid (BR) signal transduction. Phosphorylates BRI1. May be involved in changing the equilibrium between plasma membrane-located BRI1 homodimers and endocytosed BRI1-BAK1 heterodimers. Interaction with MSBP1 stimulates the endocytosis of BAK1 and suppresses brassinosteroid signaling. Acts in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) via its interaction with FLS2 and the phosphorylation of BIK1. Involved in programmed cell death (PCD) control. Positively regulates the BR-dependent plant growth pathway and negatively regulates the BR-independent cell-death pathway.<ref>PMID:17600708</ref> <ref>PMID:17583510</ref> <ref>PMID:18694562</ref> <ref>PMID:18667726</ref> <ref>PMID:19124768</ref> <ref>PMID:20018402</ref> <ref>PMID:20404519</ref> <ref>PMID:21350342</ref>
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Authors: Chai, J., Sun, Y., Han, Z.
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==About this Structure==
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[[4mn8]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MN8 OCA].
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Description: Crystal structure of flg22 in complex with the FLS2 and BAK1 ectodomains
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==Reference==
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<ref group="xtra">PMID:024114786</ref><references group="xtra"/><references/>
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[[Category: Non-specific serine/threonine protein kinase]]
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[[Category: Chai, J.]]
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[[Category: Han, Z.]]
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[[Category: Sun, Y.]]
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[[Category: Bak1]]
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[[Category: Flagellin]]
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[[Category: Flg22]]
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[[Category: Fls2]]
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[[Category: Leucine-rich repeat]]
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[[Category: Plant immunity]]
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[[Category: Transferase-transferase receptor complex]]

Revision as of 07:22, 4 December 2013

Template:STRUCTURE 4mn8

Contents

Crystal structure of flg22 in complex with the FLS2 and BAK1 ectodomains

Template:ABSTRACT PUBMED 24114786

Function

[FLS2_ARATH] Constitutes the pattern-recognition receptor (PPR) that determines the specific perception of flagellin (flg22), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). Flagellin-binding to the receptor is the first step to initiate the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6), resulting in enhanced resistance against pathogens. Binding to the effector AvrPto1 from Pseudomonas syringae blocks the downstream plant immune response.[1] [2] [3] [4] [5] [6] [BAK1_ARATH] Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Controls the expression of genes associated with innate immunity in the absence of pathogens or elicitors. Involved in brassinosteroid (BR) signal transduction. Phosphorylates BRI1. May be involved in changing the equilibrium between plasma membrane-located BRI1 homodimers and endocytosed BRI1-BAK1 heterodimers. Interaction with MSBP1 stimulates the endocytosis of BAK1 and suppresses brassinosteroid signaling. Acts in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) via its interaction with FLS2 and the phosphorylation of BIK1. Involved in programmed cell death (PCD) control. Positively regulates the BR-dependent plant growth pathway and negatively regulates the BR-independent cell-death pathway.[7] [8] [9] [10] [11] [12] [13] [14]

About this Structure

4mn8 is a 3 chain structure. Full crystallographic information is available from OCA.

Reference

  • Sun Y, Li L, Macho AP, Han Z, Hu Z, Zipfel C, Zhou JM, Chai J. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science. 2013 Nov 1;342(6158):624-8. doi: 10.1126/science.1243825. Epub 2013 Oct , 10. PMID:24114786 doi:http://dx.doi.org/10.1126/science.1243825
  1. ↑ Gomez-Gomez L, Boller T. FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis. Mol Cell. 2000 Jun;5(6):1003-11. PMID:10911994
  2. ↑ Gomez-Gomez L, Bauer Z, Boller T. Both the extracellular leucine-rich repeat domain and the kinase activity of FSL2 are required for flagellin binding and signaling in Arabidopsis. Plant Cell. 2001 May;13(5):1155-63. PMID:11340188
  3. ↑ Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, Boller T, Ausubel FM, Sheen J. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature. 2002 Feb 28;415(6875):977-83. PMID:11875555 doi:http://dx.doi.org/10.1038/415977a
  4. ↑ Zipfel C, Robatzek S, Navarro L, Oakeley EJ, Jones JD, Felix G, Boller T. Bacterial disease resistance in Arabidopsis through flagellin perception. Nature. 2004 Apr 15;428(6984):764-7. PMID:15085136 doi:http://dx.doi.org/10.1038/nature02485
  5. ↑ Gohre V, Spallek T, Haweker H, Mersmann S, Mentzel T, Boller T, de Torres M, Mansfield JW, Robatzek S. Plant pattern-recognition receptor FLS2 is directed for degradation by the bacterial ubiquitin ligase AvrPtoB. Curr Biol. 2008 Dec 9;18(23):1824-32. doi: 10.1016/j.cub.2008.10.063. PMID:19062288 doi:http://dx.doi.org/10.1016/j.cub.2008.10.063
  6. ↑ Chinchilla D, Bauer Z, Regenass M, Boller T, Felix G. The Arabidopsis receptor kinase FLS2 binds flg22 and determines the specificity of flagellin perception. Plant Cell. 2006 Feb;18(2):465-76. Epub 2005 Dec 23. PMID:16377758 doi:http://dx.doi.org/10.1105/tpc.105.036574
  7. ↑ He K, Gou X, Yuan T, Lin H, Asami T, Yoshida S, Russell SD, Li J. BAK1 and BKK1 regulate brassinosteroid-dependent growth and brassinosteroid-independent cell-death pathways. Curr Biol. 2007 Jul 3;17(13):1109-15. PMID:17600708 doi:10.1016/j.cub.2007.05.036
  8. ↑ Kemmerling B, Schwedt A, Rodriguez P, Mazzotta S, Frank M, Qamar SA, Mengiste T, Betsuyaku S, Parker JE, Mussig C, Thomma BP, Albrecht C, de Vries SC, Hirt H, Nurnberger T. The BRI1-associated kinase 1, BAK1, has a brassinolide-independent role in plant cell-death control. Curr Biol. 2007 Jul 3;17(13):1116-22. Epub 2007 Jun 21. PMID:17583510 doi:S0960-9822(07)01470-4
  9. ↑ Wang X, Kota U, He K, Blackburn K, Li J, Goshe MB, Huber SC, Clouse SD. Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in brassinosteroid signaling. Dev Cell. 2008 Aug;15(2):220-35. doi: 10.1016/j.devcel.2008.06.011. PMID:18694562 doi:10.1016/j.devcel.2008.06.011
  10. ↑ Albrecht C, Russinova E, Kemmerling B, Kwaaitaal M, de Vries SC. Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE proteins serve brassinosteroid-dependent and -independent signaling pathways. Plant Physiol. 2008 Sep;148(1):611-9. doi: 10.1104/pp.108.123216. Epub 2008 Jul, 30. PMID:18667726 doi:10.1104/pp.108.123216
  11. ↑ Oh MH, Wang X, Kota U, Goshe MB, Clouse SD, Huber SC. Tyrosine phosphorylation of the BRI1 receptor kinase emerges as a component of brassinosteroid signaling in Arabidopsis. Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):658-63. Epub 2009 Jan 5. PMID:19124768 doi:0810249106
  12. ↑ Postel S, Kufner I, Beuter C, Mazzotta S, Schwedt A, Borlotti A, Halter T, Kemmerling B, Nurnberger T. The multifunctional leucine-rich repeat receptor kinase BAK1 is implicated in Arabidopsis development and immunity. Eur J Cell Biol. 2010 Feb-Mar;89(2-3):169-74. doi: 10.1016/j.ejcb.2009.11.001., Epub 2009 Dec 16. PMID:20018402 doi:10.1016/j.ejcb.2009.11.001
  13. ↑ Lu D, Wu S, He P, Shan L. Phosphorylation of receptor-like cytoplasmic kinases by bacterial flagellin. Plant Signal Behav. 2010 May 9;5(5). PMID:20404519
  14. ↑ Oh MH, Wu X, Clouse SD, Huber SC. Functional importance of BAK1 tyrosine phosphorylation in vivo. Plant Signal Behav. 2011 Mar;6(3):400-5. Epub 2011 Mar 1. PMID:21350342

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