2r83
From Proteopedia
(New page: 200px<br /><applet load="2r83" size="350" color="white" frame="true" align="right" spinBox="true" caption="2r83, resolution 2.70Å" /> '''Crystal structure an...) |
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==Overview== | ==Overview== | ||
| - | Release of neurotransmitter from synaptic vesicles requires the | + | Release of neurotransmitter from synaptic vesicles requires the Ca2+/phospholipid-binding protein synaptotagmin 1. There is considerable evidence that cooperation between the tandem C2 domains of synaptotagmin is a requirement of regulated exocytosis; however, high-resolution structural evidence for this interaction has been lacking. The 2.7 A crystal structure of the cytosolic domains of human synaptotagmin 1 in the absence of Ca2+ reveals a novel closed conformation of the protein. The shared interface between C2A and C2B is stabilized by a network of interactions between residues on the C-terminal alpha-helix of the C2B domain and residues on loops 1-3 of the Ca2+-binding region of C2A. These interactions alter the overall shape of the Ca2+-binding pocket of C2A, but not that of C2B. Thus, synaptotagmin 1 C2A-C2B may utilize a novel regulatory mechanism whereby one C2 domain could regulate the other until an appropriate triggering event decouples them. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Fuson, K | + | [[Category: Fuson, K L]] |
[[Category: Montes, M.]] | [[Category: Montes, M.]] | ||
| - | [[Category: Robert, J | + | [[Category: Robert, J J]] |
| - | [[Category: Sutton, R | + | [[Category: Sutton, R B.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: c2a-c2b]] | [[Category: c2a-c2b]] | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:45:18 2008'' |
Revision as of 16:45, 21 February 2008
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Crystal structure analysis of human synaptotagmin 1 C2A-C2B
Overview
Release of neurotransmitter from synaptic vesicles requires the Ca2+/phospholipid-binding protein synaptotagmin 1. There is considerable evidence that cooperation between the tandem C2 domains of synaptotagmin is a requirement of regulated exocytosis; however, high-resolution structural evidence for this interaction has been lacking. The 2.7 A crystal structure of the cytosolic domains of human synaptotagmin 1 in the absence of Ca2+ reveals a novel closed conformation of the protein. The shared interface between C2A and C2B is stabilized by a network of interactions between residues on the C-terminal alpha-helix of the C2B domain and residues on loops 1-3 of the Ca2+-binding region of C2A. These interactions alter the overall shape of the Ca2+-binding pocket of C2A, but not that of C2B. Thus, synaptotagmin 1 C2A-C2B may utilize a novel regulatory mechanism whereby one C2 domain could regulate the other until an appropriate triggering event decouples them.
About this Structure
2R83 is a Single protein structure of sequence from Homo sapiens with as ligand. Known structural/functional Sites: , , , , , , and . Full crystallographic information is available from OCA.
Reference
Structure of human synaptotagmin 1 C2AB in the absence of Ca2+ reveals a novel domain association., Fuson KL, Montes M, Robert JJ, Sutton RB, Biochemistry. 2007 Nov 13;46(45):13041-8. Epub 2007 Oct 23. PMID:17956130
Page seeded by OCA on Thu Feb 21 18:45:18 2008
Categories: Homo sapiens | Single protein | Fuson, K L | Montes, M. | Robert, J J | Sutton, R B. | CL | C2a-c2b | Calcium | Cell junction | Cytoplasmic vesicle | Endocytosis | Endocytosis/exocytosis complex | Exocytosis | Glycoprotein | Lipoprotein | Membrane | Metal-binding | Palmitate | Phosphorylation | Synapse | Transmembrane
